Recombinant Proteins
Product List
TUBG1 Human
Tubulin Gamma 1 Human Recombinant
TUBG1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
TUBG1 Human, Sf9
Tubulin Gamma 1 Human Recombinant, Sf9
TUBG1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 457 amino acids (1-451 a.a.) and having a molecular mass of 51.9kDa. TUBG1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Insect cells.
Introduction
Tubulin gamma (γ-tubulin) is a member of the tubulin superfamily, which also includes α-tubulin and β-tubulin. It plays a crucial role in the nucleation and polar orientation of microtubules, primarily found in centrosomes and spindle pole bodies . γ-Tubulin is essential for the formation of the γ-tubulin ring complex (γ-TuRC), which is critical for microtubule nucleation .
Key Biological Properties: γ-Tubulin is highly conserved across eukaryotic species and is essential for microtubule organization . It is involved in the formation of spindle, phragmoplast, and cortical microtubule arrays .
Expression Patterns and Tissue Distribution: γ-Tubulin is ubiquitously expressed in eukaryotic cells, with high concentrations in centrosomes and spindle pole bodies . In plants, it is localized with all microtubule arrays and in nuclei .
Primary Biological Functions: γ-Tubulin is a key player in microtubule nucleation, which is essential for cell division and intracellular organization . It also plays a role in the coordination of late mitotic events and mitotic checkpoint control .
Role in Immune Responses and Pathogen Recognition: While γ-tubulin’s primary functions are related to microtubule organization, its role in immune responses and pathogen recognition is less well-documented. However, its involvement in cell division and intracellular transport indirectly supports immune cell functions .
Mechanisms with Other Molecules and Cells: γ-Tubulin interacts with other proteins to form the γ-tubulin ring complex (γ-TuRC), which serves as a template for microtubule nucleation . It also interacts with centrosomal proteins to organize microtubules .
Binding Partners and Downstream Signaling Cascades: γ-Tubulin binds to accessory proteins such as Spc97 and Spc98 to form the γ-tubulin small complex (γ-TuSC), which is essential for microtubule nucleation . It also interacts with proteins involved in cell cycle regulation and stress signaling .
Regulatory Mechanisms: The expression and activity of γ-tubulin are regulated by various mechanisms, including transcriptional regulation and post-translational modifications . γ-Tubulin is part of a multi-subunit ring complex that promotes microtubule nucleation and caps the minus ends of microtubules .
Transcriptional Regulation and Post-Translational Modifications: γ-Tubulin undergoes various post-translational modifications that affect its function and interactions with other proteins . These modifications include phosphorylation, which can regulate its activity and localization .
Biomedical Research: γ-Tubulin is widely studied in the context of cell division and cancer research. Its role in microtubule nucleation makes it a target for anti-cancer drugs .
Diagnostic Tools and Therapeutic Strategies: γ-Tubulin antibodies are used in diagnostic tools to study microtubule organization and cell division . Targeting γ-tubulin and its associated complexes is a potential therapeutic strategy for cancer treatment .
Role Throughout the Life Cycle: γ-Tubulin is essential for proper cell division and development. Disruption of γ-tubulin function can lead to defects in spindle formation and cell division, affecting development and growth . It also plays a role in aging and disease, particularly in cancer, where its dysregulation can contribute to tumorigenesis .
