TUBG1 Human

Gamma tubulin, specifically, is found at microtubule organizing centers (MTOCs) such as the spindle poles or the centrosome. It is a key component of the gamma-tubulin ring complex (γ-TuRC), which is essential for the nucleation of microtubules at their minus ends . This nucleation process is critical for the formation and stabilization of the microtubule network within cells.

The human recombinant form of Tubulin Gamma 1 is often produced in expression systems like E. coli for research purposes. This recombinant protein is typically tagged with His tags to facilitate purification and detection .

Gamma tubulin plays a vital role in several cellular processes:

• Microtubule Nucleation: It initiates the polymerization of tubulin dimers to form microtubules.
• Cell Cycle Progression: It is involved in the formation of the mitotic spindle, which is necessary for chromosome segregation during cell division.
• Centrosome Duplication: It helps in the duplication of centrosomes, ensuring proper cell division and genetic stability .

Recombinant Tubulin Gamma 1 is widely used in cell biology research to study microtubule dynamics, cell division, and intracellular transport. It is also used in structural biology to understand the architecture of microtubule organizing centers and the mechanisms of microtubule nucleation .

The recombinant protein is usually provided as a lyophilized powder and should be stored at -20°C to -80°C for long-term stability. Upon reconstitution, it can be stored at 4-8°C for short-term use. It is recommended to aliquot the reconstituted protein to avoid repeated freeze-thaw cycles .