TUBG1 Human, Sf9
Sf9, Insect cells.
Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1, TUBG1, TUBG, Tubulin, Gamma 1, Gamma-1-Tubulin, TUBGCP1, CDCBM4.
Description
TUBG1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 457 amino acids (1-451 a.a.) and having a molecular mass of 51.9kDa. TUBG1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1, TUBG1, TUBG, Tubulin, Gamma 1, Gamma-1-Tubulin, TUBGCP1, CDCBM4.
Sf9, Insect cells.
MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE QHHHHHH.
Product Science Overview
Tubulin Gamma 1 (TUBG1) is a member of the tubulin superfamily, which plays a crucial role in the nucleation and organization of microtubules. Microtubules are essential components of the cytoskeleton, involved in various cellular processes such as cell division, intracellular transport, and maintenance of cell shape. TUBG1 is specifically involved in the formation of the gamma-tubulin ring complex (γ-TuRC), which is critical for microtubule nucleation at the centrosome .
TUBG1 produced in Sf9 insect cells is a recombinant protein that consists of a single, glycosylated polypeptide chain containing 457 amino acids. It has a molecular mass of approximately 51.9 kDa. The recombinant protein is expressed with a 6-amino acid His tag at the C-terminus, which facilitates its purification using chromatographic techniques .
TUBG1 localizes to the centrosome and binds to microtubules to form the γ-TuRC. This complex is essential for the nucleation of microtubules, which is the initial step in microtubule formation. The γ-TuRC acts as a template for the addition of α- and β-tubulin dimers, leading to the polymerization of microtubules. TUBG1 is also involved in the progression of the cell cycle, as it ensures the proper formation and function of the mitotic spindle during cell division .
Recombinant TUBG1 produced in Sf9 insect cells is widely used in research to study the mechanisms of microtubule nucleation and organization. It is also used to investigate the role of γ-tubulin in various cellular processes, including cell division and intracellular transport. Additionally, TUBG1 can be used as a tool to identify and characterize proteins that interact with the γ-TuRC .
The recombinant TUBG1 protein is provided as a sterile, filtered, colorless solution. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 40% glycerol, 0.1 M NaCl, 2 mM DTT, and 50 mM imidazole. For short-term storage, the protein can be kept at 4°C for 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability .
