TUBG1 Antibody
Description
Product Specs
PAT9D6AT.
Product Science Overview
Tubulin Gamma 1 (TUBG1) is a protein that plays a crucial role in the formation and function of microtubules, which are essential components of the cytoskeleton in eukaryotic cells. Microtubules are involved in various cellular processes, including cell division, intracellular transport, and maintenance of cell shape. TUBG1 is a highly conserved protein found in the microtubule organizing centers (MTOCs) of eukaryotic cells .
TUBG1 is a member of the tubulin family and is encoded by the TUBG1 gene located on human chromosome 17q21.2 . The protein has a molecular weight of approximately 48 kDa and is characterized by its ability to nucleate the assembly of microtubules. This nucleation process is essential for the formation of the mitotic spindle during cell division .
The TUBG1 transcript is widely expressed in various tissues, including preimplantation embryos and the brain . The protein is predominantly localized in the centrosome, which is the primary MTOC in animal cells. In addition to its role in microtubule nucleation, TUBG1 is also involved in the regulation of microtubule dynamics and stability .
Mouse anti-human TUBG1 antibodies are monoclonal antibodies developed to specifically recognize and bind to the TUBG1 protein in human cells. These antibodies are produced by immunizing mice with human TUBG1 protein or synthetic peptides corresponding to regions of the TUBG1 protein. The resulting hybridoma cells are then screened for the production of antibodies that specifically target TUBG1 .
Mouse anti-human TUBG1 antibodies are widely used in various scientific applications, including:
- Western Blotting (WB): To detect and quantify TUBG1 protein levels in cell and tissue lysates.
- Immunocytochemistry (ICC): To visualize the localization of TUBG1 in cultured cells.
- Immunohistochemistry (IHC): To study the distribution of TUBG1 in tissue sections.
- Immunoprecipitation (IP): To isolate TUBG1 protein complexes from cell lysates for further analysis .
These antibodies are valuable tools for researchers studying the role of TUBG1 in cellular processes and its involvement in various diseases, including cancer and neurodegenerative disorders .
