Recombinant Proteins
Product List
RASSF1A Human
Ras association domain-containing protein 1 Human Recombinant
RASSF1A is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
RASSF3 Human
Ras Association Domain-Containing Protein 3 Human Recombinant
Introduction
The Ras Association Domain Family (RASSF) comprises a group of proteins characterized by the presence of a Ras-association (RA) domain. These proteins are classified into two subclasses: C-RASSF and N-RASSF. The C-RASSF proteins (RASSF1-6) contain a C-terminal coiled-coil motif known as the Salvador/RASSF/Hippo (SARAH) domain, while the N-RASSF proteins (RASSF7-10) lack this motif .
Key Biological Properties: RASSF proteins are involved in various cellular processes, including apoptosis, cell cycle regulation, and microtubule stability. They act as tumor suppressors and are frequently inactivated in cancers through promoter hypermethylation .
Expression Patterns and Tissue Distribution: RASSF proteins are expressed in various tissues, with specific expression patterns depending on the family member. For example, RASSF10 shows a striking expression pattern in the rhombencephalon (hindbrain) of developing Xenopus embryos .
Primary Biological Functions: RASSF proteins play crucial roles in regulating apoptosis, cell cycle progression, and maintaining genomic stability. They also act as scaffolding agents in microtubule stability and modulate NF-κB activity and inflammation duration .
Role in Immune Responses and Pathogen Recognition: While the primary focus of RASSF proteins is on tumor suppression and cell cycle regulation, they also have roles in modulating immune responses and pathogen recognition through their interactions with various signaling pathways .
Mechanisms with Other Molecules and Cells: RASSF proteins interact with various molecules and cells through their RA and SARAH domains. These interactions include binding to Ras GTPases and other tumor suppressors like p53 and pRb .
Binding Partners and Downstream Signaling Cascades: RASSF proteins are involved in multiple signaling cascades, including the Hippo pathway, which controls tissue size and cell proliferation. They also interact with mammalian Ste20-like kinases, contributing to their tumor suppressor functions .
Transcriptional Regulation: The expression of RASSF genes is regulated at the transcriptional level, often through promoter methylation. This epigenetic modification leads to the silencing of RASSF genes in various cancers .
Post-Translational Modifications: RASSF proteins undergo post-translational modifications, such as phosphorylation, which can affect their stability, localization, and interactions with other proteins .
Biomedical Research: RASSF proteins are extensively studied in cancer research due to their tumor suppressor functions. Understanding their roles and regulatory mechanisms can provide insights into cancer development and potential therapeutic targets .
Diagnostic Tools: The methylation status of RASSF genes, particularly RASSF1A, is used as a biomarker for cancer diagnosis and prognosis. Detecting promoter hypermethylation can help identify early-stage cancers and predict patient outcomes .
Therapeutic Strategies: Targeting the regulatory mechanisms of RASSF proteins, such as reversing promoter methylation, holds potential for developing new cancer therapies. Demethylating agents are currently being explored in clinical trials .
