Recombinant Proteins
Product List
MAGOH Human
Mago-Nashi Homolog Human Recombinant
MAGOHB Human
Mago-Nashi Homolog B Human Recombinant
MAGOHB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
The Mago-Nashi Homolog (MAGOH) is a protein encoded by the MAGOH gene in humans. It is the mammalian homolog of the Drosophila mago nashi gene, which is crucial for germplasm assembly and germline development . In mammals, MAGOH is a core component of the exon junction complex (EJC), playing a vital role in mRNA processing .
Key Biological Properties: MAGOH is an RNA-binding protein involved in various developmental processes, including germ cell differentiation . It is essential for pre-mRNA splicing as part of the spliceosome and plays a redundant role with its paralog MAGOHB in the EJC and nonsense-mediated decay (NMD) pathway .
Expression Patterns and Tissue Distribution: MAGOH is ubiquitously expressed in adult tissues and can be induced by serum stimulation of quiescent fibroblasts . It is highly expressed in oocytes, secondary oocytes, monocytes, and various other tissues .
Primary Biological Functions: MAGOH is required for mRNA splicing, export, translation, and NMD as part of the EJC . It influences downstream processes, including nuclear mRNA export, subcellular mRNA localization, translation efficiency, and NMD .
Role in Immune Responses and Pathogen Recognition: While specific roles in immune responses and pathogen recognition are not well-documented, MAGOH’s involvement in mRNA processing suggests it may indirectly influence immune functions by regulating the expression of immune-related genes.
Mechanisms with Other Molecules and Cells: MAGOH forms a stable heterodimer with RBM8A, which binds to the cleft of EIF4A3 to form the core of the EJC . The EJC is deposited upstream of each exon-exon junction, marking spliced transcripts and influencing their translation efficiency .
Binding Partners and Downstream Signaling Cascades: MAGOH interacts with RBM8A and NXF1, among other proteins . The EJC proteins regulate splicing efficiency and protect the global transcriptome by masking cryptic splice sites .
Transcriptional Regulation: MAGOH expression can be induced by serum stimulation of quiescent fibroblasts . The regulatory mechanisms controlling its expression and activity involve various transcription factors and signaling pathways.
Post-Translational Modifications: Specific post-translational modifications of MAGOH have not been extensively documented. However, as a core component of the EJC, it is likely subject to regulatory modifications that influence its function in mRNA processing.
Biomedical Research: MAGOH is a crucial component in studying mRNA processing, splicing, and NMD. Its role in these processes makes it a valuable target for understanding genetic disorders and developmental biology .
Diagnostic Tools and Therapeutic Strategies: The haploinsufficiency of MAGOH is associated with disorders such as microcephaly and cancer . Understanding its function and regulation can lead to novel diagnostic and therapeutic approaches for these conditions.
Development to Aging and Disease: MAGOH is essential for embryonic development and normal cellular functioning . Its role in mRNA processing is critical throughout the life cycle, from development to aging. Dysregulation of MAGOH can lead to various diseases, including cancer and developmental disorders .
