MAGOH Human

The Mago-Nashi homolog, often referred to as MAGOH, is a protein that plays a crucial role in various biological processes. It is the human homolog of the Drosophila mago nashi protein, which is essential for normal germplasm development in the Drosophila embryo . In humans, MAGOH is involved in the exon junction complex (EJC), which is critical for mRNA processing and regulation.

The MAGOH gene encodes a protein that consists of 146 amino acids and has a molecular weight of approximately 18 kDa . The protein is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts . The recombinant human MAGOH protein is often expressed in E. coli and purified using conventional chromatography techniques .

MAGOH is a component of the exon junction complex (EJC), which is deposited on messenger RNAs (mRNAs) during splicing . The core of the EJC consists of eIF4A3, MLN51, Y14, and MAGOH . This complex plays a vital role in mRNA surveillance and nonsense-mediated decay (NMD), a process that degrades mRNAs containing premature stop codons .

Diseases associated with MAGOH include metaphyseal chondrodysplasia, Schmid type, and primary autosomal recessive microcephaly . The protein’s involvement in mRNA processing and regulation makes it a significant target for research in various genetic disorders and developmental abnormalities.

Recombinant human MAGOH protein is typically produced with a polyhistidine tag at the C-terminus to facilitate purification . The protein is supplied in a buffer solution and is stable for up to twelve months when stored at -20°C to -80°C . It is used in various research applications to study its function and role in different biological processes.