MAGOHB Human

Mago-Nashi Homolog B Human Recombinant
Cat. No.
BT4850
Source
Escherichia Coli.
Synonyms
mago-nashi homolog B (Drosophila), mago, magoh, MGN2, MAGOHB, MAGOH2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAGOHB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (1-148 a.a.) and having a molecular mass of 19.7kDa.
MAGOHB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mago-Nashi Homolog B, also known as MAGOHB, belongs to the mago nashi family of proteins. This protein plays a crucial role in mRNA splicing and the nonsense-mediated decay (NMD) pathway, which degrades mRNAs containing premature stop codons. MAGOHB interacts with RBM8A and forms part of the exon junction complex (EJC). This complex comprises several proteins, including NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, and ALYREF/THOC4, and is involved in various aspects of mRNA metabolism.
Description
This product consists of a recombinant human MAGOHB protein produced in E. coli. It is a single, non-glycosylated polypeptide chain that contains 171 amino acids, with the first 148 amino acids corresponding to the MAGOHB sequence. The protein has a molecular mass of 19.7 kDa. For purification and detection purposes, a 23 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is provided as a clear solution that has been sterilized by filtration.
Formulation
The MAGOHB protein is supplied in a solution with a concentration of 0.25 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, and 0.15 M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the MAGOHB protein is greater than 90.0%, as assessed by SDS-PAGE analysis.
Synonyms
mago-nashi homolog B (Drosophila), mago, magoh, MGN2, MAGOHB, MAGOH2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAVASDF YLRYYVGHKG KFGHEFLEFE FRPDGKLRYA NNSNYKNDVM IRKEAYVHKS VMEELKRIID DSEITKEDDA LWPPPDRVGR QELEIVIGDE HISFTTSKIG SLIDVNQSKD PEGLRVFYYL VQDLKCLVFS LIGLHFKIKP I.

Product Science Overview

Introduction

Mago-Nashi Homolog B (MAGOHB) is a protein-coding gene that plays a crucial role in various cellular processes. It is a human homolog of the Drosophila mago nashi gene, which is essential for germline development and embryo formation. In humans, MAGOHB is ubiquitously expressed in adult tissues and is involved in several critical pathways, including mRNA splicing, export, translation, and nonsense-mediated mRNA decay (NMD) .

Gene and Protein Structure

MAGOHB is part of the exon junction complex (EJC), a multiprotein complex that assembles on mRNA after splicing. The EJC is composed of core components, including EIF4A3, RBM8A, and MAGOH/MAGOHB. These proteins form a stable heterodimer that binds to the cleft of EIF4A3, creating the core of the complex . The EJC marks the position of exon-exon junctions in mature mRNA, influencing downstream processes such as nuclear mRNA export, subcellular mRNA localization, translation efficiency, and NMD .

Biological Functions

MAGOHB is essential for normal cellular functioning and embryonic development. It plays a redundant role with its paralog, MAGOH, in the EJC and NMD pathways . The EJC is a dynamic structure that consists of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex transiently during EJC assembly or subsequent mRNA metabolism . The presence of EJC proteins regulates splicing efficiency and protects the global transcriptome by masking cryptic splice sites .

Clinical Significance

Haploinsufficiency of MAGOHB has been associated with various disorders, including microcephaly and cancer . The depletion of core EJC components, such as RBM8A, results in aberrant splicing, particularly for genes related to mitosis like AURKB (Aurora B Kinase) and MDM2 (murine double minute 2) . The emerging molecular role of the EJC is to prevent re-splicing of mRNAs and ensure proper centrosome organization and ciliogenesis .

Recombinant MAGOHB

Recombinant human MAGOHB is produced using recombinant DNA technology, which involves inserting the human MAGOHB gene into a suitable expression system, such as bacteria, yeast, or mammalian cells. This allows for the large-scale production of the protein for research and therapeutic purposes. Recombinant MAGOHB is used in various studies to understand its role in cellular processes and its potential as a target for cancer treatment .

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MAGOH Human
Mago-Nashi Homolog Human Recombinant
Cat. No.
BT4748
Source
Escherichia Coli.
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