Recombinant Proteins
Product List
F3 Human
Coagulation Factor III Human Recombinant
The Tissue factor is purified by proprietary chromatographic techniques.
F3 Mouse
Coagulation Factor III Mouse Recombinant
F7 Human
Coagulation Factor VIIa Human Recombinant
The Factor-VIIa is purified by proprietary chromatographic techniques.
F8 Human
Coagulation Factor-VIII Human
Human Factor VIII produced from Human Plasma contains 2332 amino acids and having a molecular mass of 330kDa. Factor-VIII is effective in the correction and prevention of severe bleeding episodes attributed to Factor VIII deficiency.
The Factor-VIII is purified by proprietary chromatographic techniques.
Human Plasma.
F8 Protein
Coagulation Factor-VIII Human Recombinant
Antihemophilic Facor Human Recombinant produced in CHO is a glycosylated polypeptide chain having 2322 amino acids. The Factor-VIII is purified by proprietary chromatographic techniques.
MCFD2 Human
Multiple Coagulation Factor Deficiency 2 Human Recombinant
MCFD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 136 amino acids (27-146 a.a.) and having a molecular wieght of 15.1kDa. The MCFD2 is is fused to 16 a.a. T7-Tag at N-terminus and purified by proprietary chromatographic techniques.
Introduction
Coagulation factors are a group of proteins essential for blood clotting, a critical process that prevents excessive bleeding when blood vessels are injured. These factors work in a complex cascade to form a stable blood clot. They are classified into several categories based on their function and structure:
- Proenzymes (Zymogens): Inactive precursors that are converted into active enzymes. Examples include Factor II (Prothrombin), Factor VII, Factor IX, and Factor X.
- Cofactors: Non-enzymatic proteins that assist in the activation of proenzymes. Examples include Factor V and Factor VIII.
- Fibrinogen: A soluble plasma protein that is converted into insoluble fibrin during clot formation.
- Regulatory Proteins: Proteins that regulate the coagulation process, such as Protein C, Protein S, and Antithrombin.
Key Biological Properties: Coagulation factors are primarily synthesized in the liver and circulate in the blood in an inactive form. They are activated in response to vascular injury.
Expression Patterns: The expression of coagulation factors is tightly regulated and occurs predominantly in the liver. Some factors, like Factor VIII, are also produced by endothelial cells.
Tissue Distribution: Coagulation factors are found in the plasma, with some factors also present in platelets and endothelial cells.
Primary Biological Functions: The main function of coagulation factors is to facilitate blood clotting. They achieve this by participating in a cascade of reactions that lead to the formation of a stable fibrin clot.
Role in Immune Responses: Coagulation factors also play a role in the immune response by limiting the spread of pathogens through clot formation and by interacting with immune cells.
Pathogen Recognition: Some coagulation factors can recognize and bind to pathogens, aiding in their clearance from the bloodstream.
Mechanisms with Other Molecules and Cells: Coagulation factors interact with various molecules and cells, including platelets, endothelial cells, and other plasma proteins. These interactions are crucial for the activation and regulation of the coagulation cascade.
Binding Partners: Key binding partners include calcium ions, phospholipids, and specific receptors on platelets and endothelial cells.
Downstream Signaling Cascades: The activation of coagulation factors triggers a series of downstream signaling events that culminate in the conversion of fibrinogen to fibrin, forming a stable clot.
Regulatory Mechanisms: The expression and activity of coagulation factors are regulated at multiple levels, including transcriptional regulation and post-translational modifications.
Transcriptional Regulation: The synthesis of coagulation factors is controlled by various transcription factors and signaling pathways that respond to physiological and pathological stimuli.
Post-Translational Modifications: Coagulation factors undergo several post-translational modifications, such as glycosylation and gamma-carboxylation, which are essential for their proper function.
Biomedical Research: Coagulation factors are studied extensively in biomedical research to understand their role in hemostasis and thrombosis.
Diagnostic Tools: Assays that measure the activity of coagulation factors are used to diagnose bleeding disorders and monitor anticoagulant therapy.
Therapeutic Strategies: Coagulation factors are used therapeutically to treat bleeding disorders such as hemophilia. Recombinant coagulation factors and plasma-derived concentrates are commonly used in clinical practice.
Development: Coagulation factors are essential for normal development, particularly in the formation of the vascular system.
Aging: The levels and activity of coagulation factors can change with age, contributing to an increased risk of thrombotic events in the elderly.
Disease: Dysregulation of coagulation factors is associated with various diseases, including bleeding disorders, thrombotic disorders, and inflammatory conditions.
