F3 Mouse

Coagulation Factor III is a cell surface glycoprotein encoded by the F3 gene . It is synthesized as a 294 amino acid precursor, consisting of a signal peptide (residues 1 to 28) and the mature chain (residues 29 to 294). As a type I membrane protein, it contains a transmembrane region (residues 252 to 274) and a cytoplasmic tail (residues 275 to 294) .

Upon binding to Coagulation Factor VII, the inactive Factor VII is rapidly converted into its active form, Factor VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway, leading to the formation of a blood clot . This complex also has important coagulation-independent functions, such as angiogenesis .

Recombinant Mouse Coagulation Factor III is derived from a mouse myeloma cell line (NS0) and includes a C-terminal 6-His tag for purification purposes . The recombinant protein is typically lyophilized from a filtered solution containing Tris, NaCl, and Brij-35, and can be reconstituted in sterile 50 mM Tris, pH 9.0 .

The recombinant protein is highly pure, with a purity greater than 95% as determined by SDS-PAGE under reducing conditions . It is also low in endotoxins, with levels less than 1.0 EU per 1 μg of protein . The activity of the recombinant protein is measured by its ability to activate Coagulation Factor VII, with an AC50 of less than 5 µg/mL .

Recombinant Mouse Coagulation Factor III is used in various research applications, including studies on blood coagulation, cell proliferation, and angiogenesis . It is also used as a standard in ELISA assays and in cell or tissue culture experiments .