F3 Human

Coagulation Factor III is a cell surface glycoprotein that plays a pivotal role in the initiation of the blood coagulation cascade. It functions as a high-affinity receptor and cofactor for Coagulation Factor VII. When Coagulation Factor III binds to Coagulation Factor VII, it forms a complex that catalyzes the conversion of the inactive protease Factor X into the active protease Factor Xa . This complex is essential for the initiation of thrombin formation from prothrombin, leading to blood clotting.

The protein consists of three distinct domains:

1. Extracellular domain: Responsible for binding to Coagulation Factor VII.
2. Transmembrane domain: Anchors the protein to the cell membrane.
3. Cytoplasmic domain: Involved in intracellular signaling.

The recombinant production of Coagulation Factor III involves the insertion of the human gene encoding the protein into a suitable expression system, such as a mouse myeloma cell line (NS0). The cells are then cultured to produce the protein, which is subsequently purified to achieve high purity levels (greater than 95%) and low endotoxin levels (less than 1.0 EU per 1 μg of protein) .

Recombinant Coagulation Factor III is used in various research and clinical applications, including:

• Studying the coagulation cascade: It is used to investigate the mechanisms of blood clotting and the role of different factors in the coagulation process.
• Diagnostic assays: It is employed in assays to measure the activity of Coagulation Factor VII and other components of the coagulation pathway.
• Therapeutic applications: It has potential therapeutic uses in treating bleeding disorders and managing coagulation in surgical settings.

Recombinant Coagulation Factor III is typically supplied as a filtered solution in Tris and NaCl. It is shipped with polar packs and should be stored at -20 to -70°C to maintain stability. It is important to avoid repeated freeze-thaw cycles to preserve the protein’s activity .