Thromboplastin Bovine
Bovine Lung.
Tissue factor, Coagulation factor III, Thromboplastin, CD142, TF, F3, TFA.
Description
Thromboplastin bovine native
Product Specs
Tissue factor (TF), a cell surface glycoprotein encoded by the F3 gene, is the primary initiator of blood coagulation. It serves as a high-affinity receptor for coagulation factor VII. Upon vessel injury, the TF-Factor VIIa complex activates the coagulation cascade, leading to fibrin deposition and platelet activation. This complex initiates a catalytic event, triggering the coagulation cascade through limited proteolysis. Thus, TF plays a crucial role in hemostasis. Inflammatory mediators like interleukin 1, TNF, and endotoxin can stimulate TF expression on monocytes and vascular endothelial cells, indicating its involvement in the cellular immune response. Notably, congenital TF deficiency is not described in medical literature. Adequate TF levels are vital for the survival and growth of endothelium and TF-expressing tumor cells. Conversely, aberrant TF expression within blood vessels can trigger life-threatening thrombosis in conditions such as sepsis, atherosclerosis, and cancer. Alternative splicing of TF contributes to tumor progression and is associated with increased tumor cell proliferation and angiogenesis in pancreatic cancer.
Thromboplastin, derived from bovine sources, in its natural state.
The bovine thromboplastin underwent lyophilization without the addition of any other substances.
For reconstitution of the lyophilized Bovine Thromboplastin, sterile 0.9% NaCl is recommended.
While lyophilized Bovine Thromboplastin remains stable at room temperature for up to 3 weeks, it is best stored desiccated at a temperature below -18°C. After reconstitution, Prothrombin should be stored at 4°C for 2-7 days. For extended storage, freezing at -18°C is advised. To further enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. It's important to avoid repeated freeze-thaw cycles.
Tissue factor, Coagulation factor III, Thromboplastin, CD142, TF, F3, TFA.
Bovine Lung.
Product Science Overview
Thromboplastin Bovine is a type of thromboplastin that is extracted from bovine (cow) tissues. It is used as a reagent in laboratory settings to measure the clotting time of blood. This measurement is crucial for monitoring and managing patients who are on anticoagulant therapy, such as those taking vitamin K antagonists. The use of bovine thromboplastin is preferred in some cases due to its availability and effectiveness in producing consistent results.
The use of thromboplastin in medical diagnostics dates back to the early 20th century. Initially, thromboplastin was derived from human tissues, but due to ethical and practical concerns, alternative sources were sought. Bovine tissues became a popular source for thromboplastin due to their abundance and the ease of extraction. Over the years, the methods for extracting and purifying thromboplastin from bovine tissues have been refined, leading to the production of high-quality reagents for laboratory use.
Thromboplastin Bovine functions by initiating the extrinsic pathway of blood coagulation. When thromboplastin is added to a blood sample, it interacts with calcium ions and other clotting factors to form a complex that activates Factor X. This activation leads to the conversion of prothrombin to thrombin, which then converts fibrinogen to fibrin, resulting in the formation of a blood clot. The time taken for this process to occur is measured and used to assess the clotting ability of the blood.
Thromboplastin Bovine is widely used in medical diagnostics, particularly in the monitoring of anticoagulant therapy. Patients who are on anticoagulant medications, such as warfarin, require regular monitoring to ensure that their blood clotting time is within a therapeutic range. Thromboplastin Bovine is used in prothrombin time (PT) tests, which measure the time it takes for blood to clot in the presence of thromboplastin and calcium. The results of these tests help healthcare providers adjust the dosage of anticoagulant medications to achieve optimal therapeutic outcomes.
The World Health Organization (WHO) has established guidelines for the standardization of thromboplastin reagents to ensure consistency and accuracy in laboratory testing. Thromboplastin Bovine is calibrated against international standards to assign an International Sensitivity Index (ISI), which is used to standardize the results of PT tests across different laboratories . This standardization is crucial for ensuring that patients receive consistent and accurate monitoring of their anticoagulant therapy, regardless of where the tests are performed.
