Recombinant Proteins
Product List
WFDC12 Human
WAP Four-Disulfide Core Domain 12 Human Recombinant
WFDC2 Human
WAP Four-Disulfide Core Domain 2 Recombinant Human
WAP Four-Disulfide Core Domain 2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 94 amino acids and having a molecular mass of 10.0 kDa (although migrates with an apparent molecular mass of 16.9 kDa in SDS-PAGE).
The WFDC2 is purified by proprietary chromatographic techniques.
Escherichia Coli.
Sterile Filtered White lyophilized (freeze-dried) powder.
WFDC2 Human, His
WAP Four-Disulfide Core Domain 2 Human Recombinant, His Tag
Introduction
The WAP (Whey Acidic Protein) Four-Disulfide Core Domain, also known as the WFDC domain, is a protein domain characterized by the presence of eight conserved cysteine residues that form four disulfide bonds . This domain is found in a variety of proteins, including whey acidic protein, elafin, and secretory leukocyte protease inhibitor (SLPI) . The WFDC domain is classified as a protease inhibitor and is involved in various biological processes .
Key Biological Properties: The WFDC domain proteins are known for their antiprotease, antibacterial, and immunomodulatory functions . They are ubiquitously expressed throughout the human body and play a crucial role in maintaining homeostasis .
Expression Patterns and Tissue Distribution: WFDC domain proteins are expressed in various tissues, including the lungs, reproductive organs, and skin . For example, SLPI and elafin are abundantly present in the human lung, where they protect against proteolytic damage .
Primary Biological Functions: The primary functions of WFDC domain proteins include inhibition of proteases, antimicrobial activity, and modulation of immune responses . These proteins help in maintaining the integrity of tissues by preventing excessive proteolytic activity .
Role in Immune Responses and Pathogen Recognition: WFDC domain proteins play a significant role in the immune system by recognizing and neutralizing pathogens . They are involved in the innate immune response and help in the regulation of inflammation .
Mechanisms with Other Molecules and Cells: WFDC domain proteins interact with various molecules and cells to exert their effects . For instance, they inhibit proteases by inserting an inhibitory loop into the active site of the protease, thereby blocking its activity .
Binding Partners and Downstream Signaling Cascades: These proteins bind to specific receptors on the surface of cells, triggering downstream signaling cascades that lead to immune responses and other biological effects .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of WFDC domain proteins are tightly regulated at both the transcriptional and post-translational levels . Transcriptional regulation involves various transcription factors that bind to the promoter regions of WFDC genes .
Transcriptional Regulation and Post-Translational Modifications: Post-translational modifications, such as phosphorylation and glycosylation, also play a crucial role in modulating the activity of these proteins .
Biomedical Research: WFDC domain proteins are extensively studied in biomedical research due to their diverse functions and potential therapeutic applications . They are used as biomarkers for various diseases, including cancer and inflammatory conditions .
Diagnostic Tools and Therapeutic Strategies: These proteins are being explored as diagnostic tools and therapeutic agents. For example, WFDC2 is being investigated as a biomarker for ovarian cancer .
Role Throughout the Life Cycle: WFDC domain proteins play a crucial role throughout the life cycle, from development to aging and disease . They are involved in various developmental processes and help in maintaining tissue integrity and immune function .
From Development to Aging and Disease: Aberrant expression of WFDC domain proteins is associated with various diseases, including cancer and chronic inflammatory conditions . Understanding their role in these processes can provide insights into potential therapeutic targets .
