WFDC2 Human, His

WAP Four-Disulfide Core Domain 2 Human Recombinant, His Tag
Cat. No.
BT26672
Source
Escherichia Coli.
Synonyms
WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2, HE4, WAP5, EDDM4, dJ461P17.6.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

WFDC2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (31-124) containing 104 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 11.3kDa (calculated).

Product Specs

Introduction
Belonging to the WFDC domain family, WAP four-disulfide core domain protein 2 (WFDC2) is a protease inhibitor. It exhibits effectiveness against a wide array of proteases, including aspartic, serine, and thiol proteases. WFDC2 is found in various normal tissues, such as the male reproductive system, parts of the respiratory tract, and the nasopharynx. It is suggested that WFDC2 plays a role in sperm maturation. Moreover, WFDC2 shows high expression levels in several tumor cell lines, including those from the ovary, colon, breast, lung, and kidney.
Description
Recombinant WFDC2 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 31-124. It comprises 104 amino acids, including a 10 a.a N-terminal His tag, and has a calculated molecular mass of 11.3 kDa.
Physical Appearance
White lyophilized (freeze-dried) powder after filtration.
Formulation
WFDC2 is supplied as a lyophilized powder following filtration (0.4 µm) from a 0.5 mg/ml solution in PBS buffer at pH 7.5.
Solubility

To prepare a working stock solution, it is advisable to add deionized water to achieve a concentration of approximately 0.5 mg/ml. Allow the lyophilized pellet to fully dissolve. Note that WFDC2 is not sterile. Prior to using it in cell culture, it is essential to filter the product through a suitable sterile filter.

Stability
Lyophilized WFDC2 protein should be stored at -20°C. After reconstitution, aliquot the product to prevent repeated cycles of freezing and thawing. The reconstituted protein can be kept at 4°C for a limited duration; it remains stable for up to two weeks at this temperature.
Purity
SDS-PAGE analysis indicates a purity greater than 90.0%.
Synonyms
WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2, HE4, WAP5, EDDM4, dJ461P17.6.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASEKTGVCPELQ ADQNCTQECV SDSECADNLK CCSAGCATFC SLPNDKEGSC PQVNINFPQL GLCRDQCQVD SQCPGQMKCC RNGCGKVSCV TPNF.

Product Science Overview

Introduction

WAP Four-Disulfide Core Domain 2 (WFDC2), also known as Human Epididymis Protein 4 (HE4), is a protein encoded by the WFDC2 gene. This protein is a member of the WFDC domain family, characterized by the presence of a WAP (Whey Acidic Protein) signature motif. The WFDC domain contains eight cysteines that form four disulfide bonds at the core of the protein, which is crucial for its structural stability and function .

Structure and Function

The WFDC2 protein is a small secretory protein that functions primarily as a protease inhibitor. The presence of the WAP signature motif allows it to inhibit serine-type and cysteine-type endopeptidases, which are enzymes that break down proteins . This inhibitory function is essential in various physiological processes, including inflammation, immune response, and tissue remodeling.

Expression and Clinical Significance

WFDC2 is expressed in several tissues, including the pulmonary epithelial cells and the epididymis. It plays a role in sperm maturation and has been implicated in reproductive biology . Notably, WFDC2 is also overexpressed in certain types of cancers, particularly ovarian cancer. Due to its elevated levels in ovarian cancer, WFDC2 (HE4) is used as a biomarker for the diagnosis and monitoring of this disease .

Recombinant WFDC2 (Human, His Tag)

Recombinant WFDC2 protein is produced using Escherichia coli (E. coli) expression systems. The recombinant protein is often tagged with a His (Histidine) tag to facilitate purification and detection. The His tag allows for easy purification using nickel-affinity chromatography, ensuring a high degree of purity (>95%) as determined by SDS-PAGE gel analyses .

Applications

Recombinant WFDC2 (His Tag) is used in various research applications, including:

  • Western Blotting (WB): To detect the presence of WFDC2 in biological samples.
  • Enzyme-Linked Immunosorbent Assay (ELISA): For quantifying WFDC2 levels in different samples.
  • Functional Studies: To investigate the role of WFDC2 in protease inhibition and its implications in diseases .

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<p><span lang="EN-US">Escherichia Coli.</span></p>
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