WFDC12 Human

WAP Four-Disulfide Core Domain 12 Human Recombinant
Cat. No.
BT26507
Source
Escherichia Coli.
Synonyms
WAP Four-Disulfide Core Domain 12, Putative Protease Inhibitor WAP12, Whey Acidic Protein 2, Chromosome 20 Open Reading Frame 122, Protease Inhibitor WAP2, Single WAP Motif Protein 2, WAP Four-Disulfide Core Domain Protein 12, dJ211D12.4, C20orf122, SWAM2, WAP2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

WFDC12 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 111 amino acids (24-111) and having a molecular mass of 12.1 kDa. WFDC12 is fused to a 23 amino acid His-tag at N-terminus.

Product Specs

Introduction
WFDC12, a member of the WFDC (WAP-type four-disulfide core) domain family, is characterized by its WAP signature motif. This WFDC domain, containing eight cysteine residues forming four disulfide bonds at the protein's core, acts as a protease inhibitor.
Description
Recombinant WFDC12, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 111 amino acids (residues 24-111). With a molecular weight of 12.1 kDa, it features a 23 amino acid His-tag fused at its N-terminus.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The WFDC12 solution is provided at a concentration of 1mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the product is determined to be greater than 85% using SDS-PAGE analysis.
Synonyms
WAP Four-Disulfide Core Domain 12, Putative Protease Inhibitor WAP12, Whey Acidic Protein 2, Chromosome 20 Open Reading Frame 122, Protease Inhibitor WAP2, Single WAP Motif Protein 2, WAP Four-Disulfide Core Domain Protein 12, dJ211D12.4, C20orf122, SWAM2, WAP2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSVKEGIEK AGVCPADNVR CFKSDPPQCH TDQDCLGERK CCYLHCGFKC VIPVKELEEG GNKDEDVSRP YPEPGWEAKC PGSSSTRCPQ K

Product Science Overview

Gene and Protein Structure

The WFDC12 gene is located on chromosome 20q12-q13, within the centromeric cluster of WFDC genes . The protein encoded by this gene is known for its role as a protease inhibitor, which means it can inhibit the activity of proteases, enzymes that break down proteins .

Functional Role

WFDC12 has been identified as an antibacterial protein and a putative acid-stable proteinase inhibitor . It plays a significant role in the regulation of the inflammatory response in the lung. Studies have shown that recombinant WFDC12 can inhibit cathepsin G, a type of protease, and reduce the production of pro-inflammatory cytokines such as interleukin-8 and monocyte chemotactic protein-1 in monocytic cells .

Clinical Relevance

The expression of WFDC12 has been observed in human lung tissue, and its levels are elevated in patients with acute respiratory distress syndrome (ARDS) and in healthy subjects treated with lipopolysaccharide (LPS), a component that triggers inflammation . This suggests that WFDC12 may have a protective role in lung inflammation and could be a potential therapeutic target for inflammatory lung diseases.

Research and Applications

Recombinant expression and purification of WFDC12 have been optimized in Escherichia coli, allowing for detailed studies on its antiprotease, antibacterial, and immunomodulatory activities . These properties make WFDC12 a protein of interest for further research in the fields of immunology and infectious diseases.

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WFDC2 Human, His
WAP Four-Disulfide Core Domain 2 Human Recombinant, His Tag
Cat. No.
BT26672
Source
Escherichia Coli.
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