WFDC2 Human
Escherichia Coli.
WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2, HE4, WAP5, EDDM4, dJ461P17.6.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
Description
WAP Four-Disulfide Core Domain 2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 94 amino acids and having a molecular mass of 10.0 kDa (although migrates with an apparent molecular mass of 16.9 kDa in SDS-PAGE).
The WFDC2 is purified by proprietary chromatographic techniques.
Product Specs
The protein known as WAP four-disulfide core domain protein 2 (WFDC2) acts as a protease inhibitor and is classified within the WFDC domain family. Exhibiting a broad spectrum of activity, WFDC2 effectively targets various proteases, including aspartic, serine, and thiol proteases. WFDC2 expression is observed in a range of normal tissues, encompassing the male reproductive system, specific regions of the respiratory tract, and the nasopharynx, suggesting a potential role in sperm maturation. Notably, elevated WFDC2 expression is also detected in several tumor cell lines, including those derived from ovarian, colon, breast, lung, and renal cancers.
Produced in E. coli, Recombinant Human WAP Four-Disulfide Core Domain 2 is a single-chain polypeptide that lacks glycosylation. This protein consists of 94 amino acids, resulting in a molecular mass of 10.0 kDa. However, it exhibits an apparent molecular mass of 16.9 kDa when analyzed using SDS-PAGE. The purification of WFDC2 is achieved through proprietary chromatographic techniques.
Sterile Filtered White lyophilized powder.
The protein solution was subjected to filtration at a pore size of 0.2 micrometers and subsequently lyophilized. The solution used for lyophilization was a concentrated solution prepared in phosphate-buffered saline (PBS) at a pH of 7.0.
To reconstitute the lyophilized Human WAP Four-Disulfide Core Domain 2, it is recommended to dissolve it in sterile 18 megaohm-centimeter (MΩ·cm) H2O. The initial reconstitution should be performed at a concentration of no less than 100 micrograms per milliliter (µg/ml). Following reconstitution, the solution can be further diluted using other aqueous solutions as needed.
Lyophilized WAP Four-Disulfide Core Domain 2 Human Recombinant demonstrates stability at room temperature for a period of 3 weeks. However, for extended storage, it is recommended to store the lyophilized protein in a desiccated state at a temperature below -18 degrees Celsius. Once reconstituted, WFDC2 should be stored at 4 degrees Celsius for a duration of 2 to 7 days. For long-term storage of the reconstituted protein, it is advisable to store it at temperatures below -18 degrees Celsius. To enhance stability during long-term storage, the addition of a carrier protein, such as human serum albumin (HSA) or bovine serum albumin (BSA) at a concentration of 0.1%, is recommended. It is important to avoid repeated freeze-thaw cycles to maintain protein integrity.
The purity of the protein is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.
WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2, HE4, WAP5, EDDM4, dJ461P17.6.
Escherichia Coli.
EKTGVCPELQ ADQNCTQECV SDSECADNLK CCSAGCATFC SLPNDKEGSC PQVNINFPQL GLCRDQCQVD SQCPGQMKCC RNGCGKVSCV TPNF.
Product Science Overview
WAP Four-Disulfide Core Domain 2 (WFDC2), also known as Human Epididymis Protein 4 (HE4), is a protein encoded by the WFDC2 gene in humans. This protein is a member of the WFDC domain family, characterized by the presence of a WAP (Whey Acidic Protein) signature motif. The WFDC domain contains eight cysteines forming four disulfide bonds at the core of the protein, which is crucial for its structural stability and function .
The WFDC2 protein is a small secretory protein that functions primarily as a protease inhibitor. Protease inhibitors are essential in regulating proteolytic activities, which involve the breakdown of proteins by proteases. By inhibiting these enzymes, WFDC2 helps maintain the balance of protein degradation and synthesis within the body .
One of the most notable aspects of WFDC2 is its role as a tumor marker for ovarian cancer. The protein’s expression levels are elevated in approximately 80% of ovarian cancer cases, making it a valuable diagnostic tool. The sensitivity of WFDC2 as a tumor marker is around 80% at a cut-off of 150 pmol/L .
Recombinant WFDC2 refers to the protein produced through recombinant DNA technology. This involves inserting the WFDC2 gene into a suitable expression system, such as E. coli, to produce the protein in large quantities. Recombinant WFDC2 is used in various research applications, including studying its structure, function, and potential therapeutic uses .
Recent studies have shown that WFDC2 may play a critical role in promoting metastasis in ovarian cancer. This suggests that targeting WFDC2 could be a potential therapeutic strategy for treating this type of cancer. Further research is ongoing to explore the full therapeutic potential of WFDC2 and its role in other diseases .
