Recombinant Proteins
Product List
JAM2 Human
Junctional Adhesion Molecule 2 Human Recombinant
JAM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
JAM3 Human
Junctional Adhesion Molecule 3 Human Recombinant
JAM3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
JAM2 Human, Sf9
Junctional Adhesion Molecule 2 Human Recombinant, Sf9
JAM2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 452 amino acids (29-238a.a.) and having a molecular mass of 50.7kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
JAM2 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Introduction
Junctional Adhesion Molecules (JAMs) are proteins that belong to the immunoglobulin superfamily. They are expressed in various tissues, including leukocytes, platelets, epithelial, and endothelial cells . JAMs are typically around 40 kDa in size and consist of immunoglobulin-like V-set domains followed by a second immunoglobulin domain . There are three major types of JAMs: JAM-A, JAM-B, and JAM-C, each playing distinct roles in cellular processes .
Key Biological Properties: JAMs are involved in regulating signal complex assembly on both their cytoplasmic and extracellular domains through interactions with scaffolding proteins containing PDZ domains . They form tight junctions in epithelial and endothelial cells, contributing to cell polarity .
Expression Patterns and Tissue Distribution: JAMs are expressed in a variety of tissues, including leukocytes, platelets, epithelial, and endothelial cells . They are localized at the tight junctions of polarized cells and on the cell surface of leukocytes .
Primary Biological Functions: JAMs play crucial roles in cell migration, cell polarity, and endothelium permeability . They are involved in leukocyte migration through homophilic and heterophilic interactions with other proteins .
Role in Immune Responses and Pathogen Recognition: JAMs influence leukocyte-endothelial cell interactions, which are moderated by integrins . They also play a role in immune responses by regulating the migration of leukocytes to sites of inflammation .
Mechanisms with Other Molecules and Cells: JAMs interact with integrins such as LFA-1 and Mac-1, which are contained in leukocyte β2 and α4β1 . They also interact with PDZ domain-containing scaffolding proteins like ZO-1, claudin, and afadin .
Binding Partners and Downstream Signaling Cascades: JAMs recruit protein scaffolds to specific sites of cell-cell adhesion and assemble signaling complexes at those sites . This interaction triggers intracellular signaling cascades that regulate various cellular functions .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of JAMs are regulated by transcriptional and post-translational modifications . These modifications include phosphorylation of their cytoplasmic domains, which affects their interaction with other proteins .
Transcriptional Regulation and Post-Translational Modifications: JAMs undergo various post-translational modifications, such as phosphorylation, which modulate their function and interactions with other proteins .
Biomedical Research: JAMs are used in research to study cell adhesion, migration, and signaling pathways . They are also investigated for their role in various diseases, including cancer and cardiovascular diseases .
Diagnostic Tools and Therapeutic Strategies: JAMs have potential applications in diagnostic tools and therapeutic strategies. For example, targeting JAMs can help in developing treatments for inflammatory diseases and cancer .
Role Throughout the Life Cycle: JAMs play essential roles throughout the life cycle, from development to aging and disease . They are involved in processes such as epithelial and endothelial barrier formation, hemostasis, angiogenesis, hematopoiesis, germ cell development, and nervous system development .
From Development to Aging and Disease: During development, JAMs regulate cell-cell contact formation and cell migration . In aging and disease, they are implicated in various pathological conditions, including cancer and inflammatory diseases .
