Recombinant Proteins
Product List
MOBKL1B Human
MOB1, Mps One Binder Kinase Activator-Like 1B Human Recombinant
MOBKL3 Human
MOB1, Mps One Binder kinase Activator-Like 3 Human Recombinant
Introduction
Mps One Binder Kinase Activator 1 (MOB1) is a multifunctional protein best known for its integrative role in regulating the Hippo and NDR pathway signaling in metazoans and the Mitotic Exit Network in yeast . MOB1 proteins are evolutionarily conserved eukaryotic proteins, with sizes ranging from 210 to 314 amino acids . The founding member was discovered in yeast through interaction screens with the kinase Mps1 and shown to be critical for mitotic exit .
Key Biological Properties: MOB1 is a globular scaffold protein without any known enzymatic activities . It acts as a signal transducer in essential intracellular pathways .
Expression Patterns and Tissue Distribution: MOB1 proteins are highly conserved across eukaryotes. In unicellular organisms like yeast, MOB1 proteins Mob1p and Mob2p are expressed by two independent genes . In multicellular organisms, such as flies and humans, multiple MOB proteins are encoded by different gene loci .
Primary Biological Functions: MOB1 acts as a co-activator of the NDR/LATS kinase family, playing a crucial role in the Hippo pathway, which regulates tissue growth and regeneration . It also facilitates the activation of downstream kinases by assembling both upstream and downstream kinases into a single complex .
Role in Immune Responses and Pathogen Recognition: While specific roles in immune responses and pathogen recognition are not well-documented, MOB1’s involvement in cell signaling pathways suggests it may play a role in these processes indirectly through its regulation of cell growth and apoptosis .
Mechanisms with Other Molecules and Cells: MOB1 binds both upstream kinases MST1 and MST2 and downstream AGC group kinases LATS1, LATS2, NDR1, and NDR2 . Binding of MOB1 to MST1 and MST2 is mediated by its phosphopeptide-binding infrastructure, while binding to LATS and NDR kinases is mediated by a distinct interaction surface on MOB1 .
Binding Partners and Downstream Signaling Cascades: MOB1 facilitates the activation of downstream kinases through a trans-phosphorylation event . It also associates with other signaling complexes, such as those containing the Rho guanine exchange factors and the serine/threonine phosphatase PP6 .
Expression and Activity Control: MOB1’s expression and activity are regulated through phosphorylation-dependent interactions with upstream kinases . Binding of MOB1 to its upstream partners also renders MOB1 a substrate, which serves to differentially regulate its protein interaction activities .
Transcriptional Regulation and Post-Translational Modifications: MOB1 undergoes phosphorylation, which is crucial for its interaction with other proteins and its role in signaling pathways . These post-translational modifications are essential for its function as a scaffold protein in various signaling complexes .
Biomedical Research: MOB1 is studied for its role in the Hippo pathway and its implications in cancer research . Its regulatory functions in cell growth and apoptosis make it a potential target for therapeutic strategies .
Diagnostic Tools and Therapeutic Strategies: MOB1’s involvement in key signaling pathways suggests it could be used as a biomarker for certain cancers and other diseases . Therapeutic strategies targeting MOB1 could potentially modulate its activity to control abnormal cell growth .
