B.Microti p41

Babesia Microti p41 Recombinant
Cat. No.
BT25495
Source
Sf9 insect cells.
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Babesia Microti p41 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 38kDa.

B.Microti p41 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Babesiosis, an ailment triggered by apicomplexan parasites of the Babesia genus, involves a two-host life cycle typically involving a rodent (like the white-footed mouse) and a tick from the Ixodes genus. The infection process begins when an infected tick introduces Babesia sporozoites into the rodent during a blood meal. These sporozoites then invade the rodent's red blood cells, where they multiply asexually. Within the bloodstream, some parasites develop into male and female gametes, although visually indistinguishable under a light microscope. The tick becomes the definitive host when it ingests these gametes during a subsequent blood meal. Within the tick, the gametes fuse, initiating a sporogonic cycle that produces sporozoites, thus completing the parasite's life cycle. While "large" Babesia species can be transmitted vertically (from mother tick to offspring), this is not observed in "small" Babesia species such as B. microti. Humans contract babesiosis when bitten by infected ticks, becoming accidental hosts. Similar to the rodent host, sporozoites introduced by the tick invade human red blood cells and multiply asexually. The multiplication of these blood-stage parasites causes the symptoms of babesiosis in humans. Although humans are typically dead-end hosts, transmission through contaminated blood transfusions is a documented risk.
Description
Recombinant Babesia Microti p41, synthesized in SF9 cells, is a glycosylated polypeptide chain with an estimated molecular weight of 38kDa. This protein is manufactured with a 6xHis tag attached to its N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized through filtration.
Formulation
B.Microti p41 is provided in a buffer solution containing 20mM HEPES (pH 7.6), 250mM NaCl, and 20% glycerol.
Stability
For optimal preservation, refrigerate the product at 4°C if the entire vial will be used within 2 to 4 weeks. For long-term storage, freeze at -20°C. Repeated freezing and thawing cycles should be avoided.
Purity
SDS-PAGE analysis indicates a purity level exceeding 80%.
Source
Sf9 insect cells.

Product Science Overview

Babesia Microti p41 Protein

The p41 protein of Babesia microti is a highly immunogenic protein that plays a crucial role in the parasite’s life cycle. It is a membrane-associated protein that is conserved among apicomplexan hemoprotozoa, including members of the genera Babesia, Plasmodium, and Theileria . The p41 protein is involved in the invasion of host erythrocytes, a critical step for the parasite’s survival and replication within the host .

Recombinant p41 Protein

The recombinant p41 protein is produced through prokaryotic expression systems, where the gene encoding the p41 protein is cloned and expressed in bacteria such as Escherichia coli. The recombinant protein is then purified for further studies . This recombinant form of the p41 protein is used in various research applications, including the development of diagnostic assays and potential vaccines .

Immunological Significance

The p41 protein of Babesia microti has been identified as a highly seroactive antigen, meaning it elicits a strong immune response in infected hosts . Immunofluorescence assays have confirmed that the p41 protein is localized in the cytoplasm of the parasite and is specifically recognized by the sera of mice infected with Babesia microti . This strong immunogenicity makes the p41 protein a promising candidate for the development of vaccines against babesiosis .

Potential Vaccine Candidate

Studies have shown that mice immunized with the recombinant p41 protein have a decreased parasite burden after infection with Babesia microti . Passive immunization with antisera against the p41 protein has also been shown to protect mice against Babesia microti infection to a certain extent . These findings suggest that the p41 protein could serve as a novel broad-spectrum parasite vaccine candidate, providing protection against babesiosis and potentially other related infections .

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