DCUN1D2 Human

DCN1 Defective in Cullin Neddylation 1 Domain Containing 2 Human Recombinant
Cat. No.
BT28893
Source
Escherichia Coli.
Synonyms

DCN1-like protein 2, DCUN1 domain-containing protein 2, Defective in cullin neddylation protein 1-like protein 2, DCUN1D2, C13orf17, DCUN1L2.

Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
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Description

DCUN1D2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 279 amino acids (1-259 a.a.) and having a molecular mass of 32.3kDa.
DCUN1D2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DCN1-like protein 2 (DCUN1D2) is a protein that may play a role in the neddylation of cullins, which regulate SCF-type ubiquitin ligase complexes. DCUN1D2 contains one DCUN1 domain and one UBA-like domain. The gene encoding DCUN1D2 is located on human chromosome 13, which also contains major tumor suppressor genes such as BRCA2 and RB1.
Description
Recombinant DCUN1D2 is a single, non-glycosylated polypeptide chain produced in E. coli. It contains 279 amino acids (with amino acids 1-259 of the DCUN1D2 sequence), has a molecular mass of 32.3 kDa, and is fused to a 20 amino acid His-tag at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered clear solution.
Formulation
DCUN1D2 protein solution at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 1mM DTT, 30% glycerol, and 0.1M NaCl.
Stability
The product can be stored at 4°C for 2-4 weeks. For longer-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% purity as determined by SDS-PAGE.
Synonyms

DCN1-like protein 2, DCUN1 domain-containing protein 2, Defective in cullin neddylation protein 1-like protein 2, DCUN1D2, C13orf17, DCUN1L2.

Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MHKLKSSQKD KVRQFMACTQ AGERTAIYCL TQNEWRLDEA TDSFFQNPDS LHRESMRNAV DKKKLERLYG RYKDPQDENK IGVDGIQQFC DDLSLDPASI SVLVIAWKFR AATQCEFSRK EFLDGMTELG CDSMEKLKAL LPRLEQELKD TAKFKDFYQF
TFTFAKNPGQ KGLDLEMAVA YWKLVLSGRF KFLDLWNTFL MEHHKRSIPR DTWNLLLDFG NMIADDMSNY DEEGAWPVLI DDFVEYARPV VTGGKRSLF.

Product Science Overview

Gene and Protein Information
  • Gene Name: DCUN1D2 (Defective in Cullin Neddylation 1 Domain Containing 2)
  • Aliases: DCNL2, C13orf17, DCN1-Like Protein 2
  • Protein Name: DCN1 Defective in Cullin Neddylation 1 Domain Containing 2
  • Molecular Mass: Approximately 32.3 kDa
  • Amino Acids: 279 amino acids with a 20 amino acid His-tag at the N-terminus
Function and Mechanism

DCUN1D2 is part of an E3 ubiquitin ligase complex that promotes the neddylation of cullin-RING ubiquitin ligase complexes. Neddylation is a post-translational modification process that involves the conjugation of the ubiquitin-like protein NEDD8 to target proteins, which is crucial for their activation and function.

The primary function of DCUN1D2 includes:

  1. Cullin Neddylation: DCUN1D2 facilitates the transfer of NEDD8 from N-terminally acetylated NEDD8-conjugating E2 enzymes to different cullin C-terminal domain-RBX complexes .
  2. Regulation of SCF Complexes: It plays an essential role in the regulation of SCF (SKP1-CUL1-F-box protein)-type complexes, which are involved in various cellular processes, including cell cycle regulation and signal transduction .
  3. Oncogenic Potential: DCUN1D2 acts as an oncogene, promoting carcinogenic progression and malignant transformation .
Biological Pathways and Diseases

DCUN1D2 is involved in several biological pathways, including:

  • Metabolism of Proteins: It contributes to the regulation of protein metabolism through its role in neddylation .
  • Class I MHC Mediated Antigen Processing and Presentation: It is implicated in the immune response by influencing antigen processing and presentation .

Diseases associated with DCUN1D2 include:

  • Cataract: Mutations or dysregulation of DCUN1D2 have been linked to the development of cataracts .
  • Cancer: Given its role as an oncogene, DCUN1D2 is associated with various cancers, including squamous cell carcinoma .
Research and Applications

Recombinant DCUN1D2 protein is produced in E. coli and is used in various research applications to study its function and role in neddylation. The recombinant protein is available in different quantities and is shipped with ice packs to maintain its stability .

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DCUN1D1 Human
DCN1 Defective in Cullin Neddylation 1 Domain Containing 1 Human Recombinant
Cat. No.
BT28795
Source
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