DCTN6 Human

Dynactin 6 Human Recombinant
Cat. No.
BT140
Source
Escherichia Coli.
Synonyms
Dynactin 6, WS3, Dynactin Subunit P27, Protein WS-3, P27, Novel RGD-Containing Protein, Dynactin Subunit 6, WS-3, Dynactin subunit 6.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DCTN6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-190 a.a) and having a molecular mass of 23.1kDa.
DCTN6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Dynactin 6, also known as DCTN6, is a protein belonging to the dynactin subunits 5/6 family. This protein is characterized by an RGD (Arg-Gly-Asp) motif located in its N-terminal region, which is known to contribute to the adhesive properties of large proteins like fibronectin. DCTN6 shares significant sequence similarity with its mouse counterpart, which has been implicated in the process of mitochondrial biogenesis. However, the precise biological function of DCTN6 remains to be fully elucidated.
Description
Recombinant human DCTN6, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 213 amino acids (specifically, amino acids 1 to 190). It has a molecular mass of 23.1 kDa. The protein is engineered with a 23 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The DCTN6 protein is supplied in a solution with a concentration of 1 mg/ml. The solution also contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. It is important to avoid repeated cycles of freezing and thawing.
Purity
The purity of the DCTN6 protein is greater than 85.0%, as determined by SDS-PAGE analysis.
Synonyms
Dynactin 6, WS3, Dynactin Subunit P27, Protein WS-3, P27, Novel RGD-Containing Protein, Dynactin Subunit 6, WS-3, Dynactin subunit 6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEKTQK SVKIAPGAVV CVESEIRGDV TIGPRTVIHP KARIIAEAGP IVIGEGNLIE EQALIINAYP DNITPDTEDP EPKPMIIGTN NVFEVGCYSQ AMKMGDNNVI ESKAYVGRNV ILTSGCIIGA CCNLNTFEVI PENTVIYGAD CLRRVQTERP QPQTLQLDFL MKILPNYHHL KKTMKGSSTP VKN.

Product Science Overview

Introduction

Dynactin 6, also known as DCTN6, is a protein-coding gene that plays a crucial role in cellular processes. It is a subunit of the dynactin complex, which is essential for the function of the molecular motor protein dynein. Dynein is responsible for minus-end directed transport along microtubules, a critical aspect of intracellular motility.

Structure and Function

The protein encoded by the DCTN6 gene contains an RGD (Arg-Gly-Asp) motif in the N-terminal region. This motif confers adhesive properties to macromolecular proteins like fibronectin . Dynactin 6 shares a high degree of sequence similarity with its mouse homolog, which has been suggested to play a role in mitochondrial biogenesis .

Dynactin 6 is part of the dynactin complex that activates dynein for ultra-processive transport along microtubules . This complex is essential for various cellular processes, including the transport of organelles, vesicles, and other cargoes within the cell.

Biological Significance

The dynactin complex, including Dynactin 6, is vital for the proper functioning of dynein. Dynein-dynactin complexes are fast and processive motors that move along microtubules in cells . These complexes are involved in the organization, positioning, and motility of organelles, mRNA, and proteins within the cell .

Clinical Relevance

Mutations or dysregulation of the DCTN6 gene can lead to various diseases. For example, diseases associated with DCTN6 include Arthrogryposis, Distal, Type 1B, and Intellectual Developmental Disorder, Autosomal Recessive 24 . Understanding the function and regulation of Dynactin 6 is crucial for developing potential therapeutic strategies for these conditions.

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