FBXO6 Human

F-Box Protein 6 Human Recombinant
Cat. No.
BT8472
Source
Escherichia Coli.
Synonyms
FBG2, FBS2, FBX6, Fbx6b, F-box only protein 6, F-box protein that recognizes sugar chains 2, F-box/G-domain protein 2, FBXO6.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FBXO6 Human Recombinant produced in E. coli is a single polypeptide chain containing 316 amino acids (1-293) and having a molecular mass of 36.3kDa. 

FBXO6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
F-Box Protein 6, also known as FBXO6, belongs to the F-box protein family, characterized by an approximately 40 amino acid motif called the F-box. These proteins constitute one of the four subunits within the ubiquitin protein ligase complex known as SCFs (SKP1-cullin-F-box), which plays a role in phosphorylation-dependent ubiquitination. F-box proteins are categorized into three groups: Fbws with WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs possessing diverse protein-protein interaction modules or lacking recognizable motifs. FBXO6 falls under the Fbxs category, and its C-terminal region shares significant similarity with rat NFB42 (neural F Box 42 kDa), which participates in cell cycle regulation.
Description

Recombinant FBXO6 protein, of human origin, has been produced in an E. coli expression system. This results in a single polypeptide chain consisting of 316 amino acids (residues 1-293) with a molecular weight of 36.3 kDa.

The FBXO6 protein has been engineered to include a 23 amino acid His-tag at the N-terminus to facilitate purification using proprietary chromatographic methods.

Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The FBXO6 solution is supplied at a concentration of 1mg/ml and is formulated in a buffer containing 20mM Tris-HCl at a pH of 8.0, 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the entire vial can be stored at 4°C. For extended storage, it is recommended to store the protein in frozen aliquots at -20°C.
To ensure stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable.
Repeated freezing and thawing of the protein should be avoided.
Purity
Purity of the protein is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
FBG2, FBS2, FBX6, Fbx6b, F-box only protein 6, F-box protein that recognizes sugar chains 2, F-box/G-domain protein 2, FBXO6.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDAPHSK AALDSINELP ENILLELFTH VPARQLLLNC RLVCSLWRDL IDLMTLWKRK CLREGFITKD WDQPVADWKI FYFLRSLHRN LLRNPCAEED MFAWQIDFNG GDRWKVESLP GAHGTDFPDP KVKKYFVTSY EMCLKSQLVD LVAEGYWEEL

LDTFRPDIVV KDWFAARADC GCTYQLKVQL ASADYFVLAS FEPPPVTIQQ WNNATWTEVS YTFSDYPRGV RYILFQHGGR DTQYWAGWYG PRVTNSSIVV SPKMTRNQAS SEAQPGQKHG QEEAAQSPYR AVVQIF.

Product Science Overview

Structure and Function

FBXL6 contains leucine-rich repeats (LRRs) that are involved in protein-protein interactions . The primary role of FBXL6 is to act as a substrate-recognition component of the SCF-type E3 ubiquitin ligase complex . This complex is crucial for targeting specific proteins for ubiquitination and subsequent proteasomal degradation, thereby regulating various cellular processes .

Recombinant Production

Recombinant Human FBXL6 is typically produced using an in vitro wheat germ expression system . This method helps preserve the correct conformational folding necessary for the protein’s biological function . The recombinant protein often includes a GST tag at the N-terminal, which aids in purification and detection .

Applications

Recombinant FBXL6 is used in various applications, including:

  • ELISA (Enzyme-Linked Immunosorbent Assay): For detecting and quantifying specific proteins.
  • Affinity Purification: To isolate proteins or other molecules from complex mixtures.
  • Microarray: For studying gene expression or protein interactions.
  • Western Blot: For detecting specific proteins in a sample .
Storage and Stability

The recombinant FBXL6 protein is typically stored at -80°C to maintain its stability and avoid freeze-thaw cycles . It is formulated in a buffer containing 50 mM Tris-HCl and 10 mM reduced Glutathione, pH 8.0 .

Biological Significance

FBXL6 plays a significant role in regulating protein stability and gene expression by targeting specific proteins for degradation . This regulation is essential for maintaining cellular homeostasis and responding to various cellular signals .

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Source
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