LBP Human
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE.
Attention: His-tag has no protease site and can not be split off.
Product Specs
Recombinant Human LBP is provided as a lyophilized powder, prepared from a solution containing 0.3mg/ml of LBP in phosphate-buffered saline (PBS) at pH 7.2.
To reconstitute the lyophilized recombinant human LBP, add 33µl of sterile water to every 10µg of the powder. This solution can be further diluted using PBS or other suitable buffers as needed.
Recombinant Human LBP demonstrates biological activity by mediating the binding of FITC-labeled LPS (at a concentration of 0.5µg/ml) to CD14-expressing CHO cells. This activity is detectable at LBP concentrations up to 0.2µg/ml and can be measured using flow cytometry (FACS).
Product Science Overview
Lipopolysaccharide (LPS), also known as endotoxin, is a crucial component of the outer membrane of Gram-negative bacteria. It plays a vital role in maintaining the structural integrity of the bacteria and protecting them from environmental stress . LPS is highly conserved among Gram-negative bacteria and is a potent inducer of inflammatory responses in humans .
LPS is a heat-stable amphiphilic molecule composed of three distinct regions:
- Lipid A: The lipid A portion is the hydrophobic anchor of LPS and is responsible for its immunostimulatory properties. The structure of lipid A varies among bacterial species, and the number of acyl chains (fatty acids) determines the immunostimulatory capacity of LPS .
- Core Polysaccharide: This region connects lipid A to the O-antigen and consists of a short chain of sugars.
- O-antigen: The O-antigen is a repetitive glycan polymer that extends outward from the core polysaccharide. It is highly variable among different bacterial species and contributes to the antigenic diversity of LPS .
The immune system recognizes LPS through the toll-like receptor 4 (TLR4), which is a membrane-bound receptor. TLR4 detects extracellular LPS and initiates an immune response . Additionally, recent research has identified other sensors, such as transient receptor potential (TRP) channels and caspase-4/5 (and caspase-11 in mice), which recognize LPS within the cytoplasm .
Human recombinant LPS is a synthetic form of LPS produced using recombinant DNA technology. This allows for the production of LPS with specific structural characteristics, which can be used in research and therapeutic applications. Human recombinant LPS models are valuable tools for studying the mechanisms of inflammation and testing new therapeutic strategies in a controlled environment .
Human recombinant LPS is widely used in research to study the immune response and the pathogenesis of diseases such as sepsis and acute respiratory distress syndrome (ARDS). By administering small quantities of LPS to healthy volunteers, researchers can trigger an inflammatory response and gain insights into the early events of inflammation . This helps in identifying potential therapeutic targets and improving outcomes in critical illnesses .
