LBP Mouse
Lipopolysaccaride Mouse Recombinant
Cat. No.
BT3378
Source
Chinese Hamster Ovarian Cells (CHO).
Synonyms
Lipopolysaccharide-binding protein, LBP, Ly88.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE.
Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off.
Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off.
Product Specs
Introduction
Lipopolysaccharides (LPS), found on the outer membrane of Gram-negative bacteria, are a type of glycolipid. Lipopolysaccharide binding protein (LBP) is a plasma protein that facilitates the movement of bacterial LPS (endotoxin). LBP plays a role in the acute-phase immune response to Gram-negative bacterial infections. Working with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, potentially playing a role in regulating monocyte responses to LPS. LBP belongs to a family of proteins with structural and functional similarities, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Located on chromosome 20, the LBP gene is positioned directly downstream of the BPI gene. LBP facilitates the transfer of LPS monomers from LPS aggregates to HDL particles, phospholipid bilayers, and a binding site on soluble CD14 (sCD14). Acting as a soluble "shuttle" for this insoluble lipid, sCD14 can accelerate the transfer process by receiving an LPS monomer from an LPS aggregate and then transferring it to an HDL particle.
Description
Recombinant Mouse LBP is produced in a serum-free medium using mouse LBP transfected CHO-cells. For transfection purposes, the complete mouse LBP cDNA was first amplified via PCR and then cloned into the pPOL-DHFR expression vector. Rm LBP production was facilitated by a his-tag through metal affinity purification with Talon, and the process was monitored using SDS-PAGE. The analysis reveals a 58kDa band on the SDS-PAGE. Please note: The His-tag located at the LBP's c-terminal end does not have a protease site and cannot be cleaved.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Recombinant Mouse LBP was lyophilized from a protein solution with a concentration of 1 mg/ml in phosphate-buffered saline at a pH of 7.2.
Solubility
For reconstitution of the lyophilized LBP, sterile 18MΩ-cm H2O is recommended at a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized Recombinant Mouse LBP, while stable at room temperature for a period of 3 weeks, should ideally be stored desiccated at a temperature below -18°C. Once reconstituted, LBP should be stored at 4°C for 2-7 days. For long-term storage, it should be kept at a temperature below -18°C. Repeated freeze-thaw cycles should be avoided.
Biological Activity
LBP, at concentrations up to 2 µg/ml, facilitates the binding of FITC-LPS (0.5 µg/ml) to CD14+CHO transfectants at a concentration of 2 x 106/ml.
Synonyms
Lipopolysaccharide-binding protein, LBP, Ly88.
Source
Chinese Hamster Ovarian Cells (CHO).
Product Science Overview
Structure and Function
- Lipid A: This is the hydrophobic anchor of LPS and is responsible for its endotoxic properties. It is the most conserved part of LPS and is crucial for the molecule’s ability to trigger an immune response .
- Core Polysaccharide: This region connects lipid A to the O-antigen and is less conserved than lipid A. It plays a role in the stability and function of LPS .
- O-antigen: This is the most variable part of LPS and extends outward from the bacterial surface. It is involved in interactions with the host immune system and can vary significantly between different bacterial strains .
Immune Response
LPS is recognized by the immune system as a pathogen-associated molecular pattern (PAMP). It is detected by toll-like receptor 4 (TLR4) on the surface of immune cells, leading to the activation of inflammatory pathways . This recognition is crucial for the immune system to mount a defense against Gram-negative bacterial infections .
Recombinant LPS in Research
Recombinant LPS, such as that derived from mice, is used extensively in research to study immune responses and inflammatory processes. By using recombinant LPS, researchers can ensure consistency and reproducibility in their experiments . This is particularly important in studies involving the activation of TLR4 and the downstream signaling pathways .
Applications
- Immunology Research: Recombinant LPS is used to study the mechanisms of immune activation and the role of TLR4 in recognizing bacterial infections .
- Drug Development: It is used in the development of new therapeutics targeting inflammatory diseases and sepsis .
- Vaccine Research: LPS is also studied for its potential use as an adjuvant in vaccines to enhance immune responses .
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