LBP Human, HEK

Lipopolysaccarid Binding Protein, Human Recombinant, HEK
Cat. No.
BT3318
Source

HEK293 Cells.

Synonyms

Lipopolysaccharide-binding protein, LBP, MGC22233, Ly88.

Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs
In Stock
Quick Inquiry

Description

LBP Human Recombinant is a single, glycosylated polypeptide chain containing 462 amino acids (26-481a.a) and having a molecular mass of 51.7kDa (calculated). LBP is fused to a 6 a.a His tag at C-terminal.

Product Specs

Introduction
Lipopolysaccharides (LPS) are glycolipids found on the outer membrane of Gram-negative bacteria. They act as endotoxins, triggering immune responses. Lipopolysaccharide binding protein (LBP) is a plasma protein that binds to LPS and modulates the body's reaction to it. LBP facilitates the interaction of LPS with immune cells, contributing to the inflammatory response against bacterial infections. It works in conjunction with other proteins like bactericidal permeability-increasing protein (BPI) and CD14, a receptor on immune cells, to regulate LPS signaling and eliminate bacterial threats.
Description
Recombinant human LBP is a purified protein produced in the laboratory. This single-chain protein consists of 462 amino acids (specifically amino acids 26-481 of the native LBP sequence) and has a molecular weight of 51.7 kDa. To facilitate purification and research applications, a 6-amino acid histidine tag is added to the C-terminus of the protein.
Physical Appearance
White powder, freeze-dried.
Formulation
The LBP protein is purified by filtration (using a 0.4 µm filter) and then lyophilized (freeze-dried) to preserve its stability. The lyophilized product is prepared in a solution containing phosphate-buffered saline (PBS) at pH 7.5 and 5% trehalose (w/v), which acts as a stabilizing agent.
Solubility
To create a working solution, add deionized water to the lyophilized LBP powder. A recommended starting concentration is 0.5 mg/ml. Ensure the pellet fully dissolves before use.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstituting the protein, divide it into smaller portions (aliquots) and store them separately to prevent repeated freeze-thaw cycles that can damage the protein. Reconstituted LBP is stable at 4°C for a limited time; studies show no change in its properties after two weeks at this temperature.
Purity
The purity of this LBP protein is greater than 95%, as determined by SDS-PAGE, a common technique for analyzing protein purity.
Synonyms

Lipopolysaccharide-binding protein, LBP, MGC22233, Ly88.

Source

HEK293 Cells.

Amino Acid Sequence

ANPGLVARIT DKGLQYAAQE GLLALQSELL RITLPDFTGD LRIPHVGRGR YEFHSLNIHS CELLHSALRP VPGQGLSLSI SDSSIRVQGR WKVRKSFFKL QGSFDVSVKG ISISVNLLLG SESSGRPTVT ASSCSSDIAD VEVDMSGDLG WLLNLFHNQI ESKFQKVLES RICEMIQKSV SSDLQPYLQT LPVTTEIDSF ADIDYSLVEA PRATAQMLEV MFKGEIFHRN HRSPVTLLAA VMSLPEEHNK MVYFAISDYV FNTASLVYHE EGYLNFSITD DMIPPDSNIR LTTKSFRPFV PRLARLYPNM NLELQGSVPS APLLNFSPGN LSVDPYMEID AFVLLPSSSK EPVFRLSVAT NVSATLTFNT SKITGFLKPG KVKVELKESK VGLFNAELLE ALLNYYILNT FYPKFNDKLA EGFPLPLLKR VQLYDLGLQI HKDFLFLGAN VQYMRVHHHH HH.

Product Science Overview

Structure and Function

LBP is a glycoprotein that binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), which are components of the outer membrane of Gram-negative bacteria . This binding is essential for the immune system to recognize and respond to bacterial infections. LBP acts as an affinity enhancer for CD14, facilitating its association with LPS and promoting the release of cytokines in response to bacterial lipopolysaccharide .

Expression and Purity

Recombinant Human LBP is often expressed in HEK 293 cells, a human embryonic kidney cell line. This expression system is chosen for its ability to produce high-quality, biologically active proteins. The recombinant LBP typically has a purity of over 95% and an endotoxin level of less than 1 EU/µg, making it suitable for various applications such as SDS-PAGE and HPLC .

Applications

LBP is used in various research applications, including:

  • SDS-PAGE: For analyzing the protein’s purity and molecular weight.
  • HPLC: For high-resolution separation and analysis of the protein.
  • sELISA: For quantifying the protein in different samples .
Biological Activity

LBP plays a pivotal role in the innate immune response by binding to LPS and transferring it to its receptor, CD14. This interaction leads to an active inflammatory response, which is crucial for fighting bacterial infections . Additionally, LBP acts as an opsonin, enhancing the phagocytosis of Gram-negative bacteria by neutrophils and granulocytes .

Clinical Significance

High concentrations of LBP in serum have been associated with sepsis, a severe and often fatal response to bacterial infection. Recombinant human LBP has been shown to decrease the effects of LPS in sepsis serum, supporting its potential therapeutic role in managing sepsis .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

LBP Human
Lipopolysaccaride Human Recombinant
Cat. No.
BT3146
Source
Chinese Hamster Ovarian Cells (CHO).
LBP Mouse
Lipopolysaccaride Mouse Recombinant
Cat. No.
BT3378
Source
Chinese Hamster Ovarian Cells (CHO).
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.