CHMP5 Human

Charged Multivesicular Body Protein 5 Human Recombinant
Cat. No.
BT7496
Source
E.coli.
Synonyms
Charged Multivesicular Body Protein 5, SNF7 Domain-Containing Protein 2, Chromosome 9 Open Reading Frame 83, Chromatin-Modifying Protein 5, Apoptosis-Related Protein PNAS-2, Vacuolar Protein Sorting-Associated Protein 60, Chromatin Modifying Protein 5, HSPC177, PNAS-2, HVps60, SNF7DC2, CGI-34, C9orf83.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CHMP5 Human Recombinant produced in E. coli is a single polypeptide chain containing 243 amino acids (1-219) and having a molecular mass of 27.0 kDa.
CHMP5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) family, Charged Multivesicular Body Protein 5 (CHMP5) is involved in the ESCRT-III complex (endosomal sorting complex required for transport III). The ESCRT-III complex plays a crucial role in degrading surface receptor proteins and developing endocytic multivesicular bodies (MVBs). Notably, some CHMPs, like CHMP1A, exhibit both nuclear and cytoplasmic/vesicular distributions and are essential for MVB formation and cell cycle progression regulation.
Description
Recombinant CHMP5, of human origin, is produced in E. coli. It is a single polypeptide chain with a molecular weight of 27.0 kDa, comprising 243 amino acids (1-219). The protein includes a 24 amino acid His-tag fused at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The CHMP5 solution has a concentration of 0.25 mg/mL and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For longer storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of the CHMP5 is determined to be greater than 80% using SDS-PAGE analysis.
Synonyms
Charged Multivesicular Body Protein 5, SNF7 Domain-Containing Protein 2, Chromosome 9 Open Reading Frame 83, Chromatin-Modifying Protein 5, Apoptosis-Related Protein PNAS-2, Vacuolar Protein Sorting-Associated Protein 60, Chromatin Modifying Protein 5, HSPC177, PNAS-2, HVps60, SNF7DC2, CGI-34, C9orf83.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSH MNRLFG KAKPKAPPPS LTDCIGTVDS RAESIDKKIS RLDAELVKYK DQIKKMREGP AKNMVKQKAL RVLKQKRMYE QQRDNLAQQS FNMEQANYTI QSLKDTKTTV DAMKLGVKEM KKAYKQVKID QIEDLQDQLE DMMEDANEIQ EALSRSYGTP ELDEDDLEAE LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI PAS

Product Science Overview

Gene and Protein Structure

The CHMP5 gene is located on chromosome 9p13.3 and encodes a small coiled-coil protein . The protein is primarily cytosolic and localizes to vesicles near the nucleus . It is involved in the formation of intraluminal vesicles (ILVs) within MVBs, which are generated by invagination and scission from the limiting membrane of the endosome .

Function and Mechanism

CHMP5 is a probable peripherally associated component of the ESCRT-III complex, which is essential for the sorting of endosomal cargo proteins into MVBs . The MVB pathway requires the sequential function of ESCRT-O, -I, -II, and -III complexes . The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses like HIV-1 .

Biological Significance

CHMP5 is involved in various cellular processes, including:

  • Degradation of Membrane Proteins: It enables the degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes, and lipids .
  • Regulation of Cell Cycle Progression: CHMP5, along with other CHMPs, has both nuclear and cytoplasmic/vesicular distributions and is required for MVB formation and regulation of cell cycle progression .
  • Interaction with Other Proteins: CHMP5 interacts with several proteins, including SMARCA4, which may be involved in chromatin remodeling, and the SUMO-conjugating enzyme UBE2I .
Clinical Relevance

Mutations or dysregulation of CHMP5 have been associated with various diseases, including:

  • Alagille Syndrome 1: A genetic disorder that affects the liver, heart, and other parts of the body .
  • Wolfram Syndrome 2: A rare genetic disorder characterized by diabetes insipidus, diabetes mellitus, optic atrophy, and deafness .
Research and Applications

Recombinant CHMP5 is used in research to study its role in the ESCRT-III complex and its involvement in various cellular processes. It is also used to investigate the mechanisms underlying diseases associated with CHMP5 dysregulation.

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THE BIOTEK
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