EDAR Human, Sf9
Sf9, Baculovirus cells.
Ectodysplasin A Receptor, Ectodysplasin 1, Anhidrotic Receptor, Anhidrotic Ectodysplasin Receptor 1, Ectodermal Dysplasia Receptor, Downless Homolog, EDA-A1 Receptor, DL, Tumor Necrosis Factor Receptor Superfamily Member EDAR, Downless, Mouse, Homolog Of, Ectodysplasin-A Receptor, ECTD10A, ECTD10B, EDA-A1R, EDA1R, ED1R, EDA3, HRM1, ED5, ED3.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
EDAR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 413 amino acids (27-187a.a.) and having a molecular mass of 45.6kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
EDAR is expressed with a 249 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Ectodysplasin A Receptor, Ectodysplasin 1, Anhidrotic Receptor, Anhidrotic Ectodysplasin Receptor 1, Ectodermal Dysplasia Receptor, Downless Homolog, EDA-A1 Receptor, DL, Tumor Necrosis Factor Receptor Superfamily Member EDAR, Downless, Mouse, Homolog Of, Ectodysplasin-A Receptor, ECTD10A, ECTD10B, EDA-A1R, EDA1R, ED1R, EDA3, HRM1, ED5, ED3.
Sf9, Baculovirus cells.
ADPEYSNCGE NEYYNQTTGL CQECPPCGPG EEPYLSCGYG TKDEDYGCVP CPAEKFSKGG YQICRRHKDC EGFFRATVLT PGDMENDAEC GPCLPGYYML ENRPRNIYGM VCYSCLLAPP NTKECVGATS GASANFPGTS GSSTLSPFQH AHKELSGQGH LATAAAAFES ACSLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH.
Product Science Overview
The Ectodysplasin A Receptor (EDAR) is a crucial protein in human biology, encoded by the EDAR gene. It is a cell surface receptor for ectodysplasin A (EDA), a member of the tumor necrosis factor (TNF) family of ligands. EDA plays a significant role in the development of ectodermal tissues, such as skin, hair, teeth, and sweat glands .
EDA exists in two main splice variants: EDA-A1 and EDA-A2. EDA-A1 binds to EDAR, while EDA-A2 binds to a different receptor known as EDA2R or X-linked ectodermal dysplasia receptor (XEDAR) . The binding of EDA-A1 to EDAR triggers the recruitment of the intracellular EDAR-associated death domain (EDARADD) adapter protein, leading to the activation of the NF-κB signaling pathway .
The EDA/EDAR signaling pathway is essential for the formation of various ectodermal derivatives during prenatal development. Mutations in the EDA gene or its receptors can result in hypohidrotic ectodermal dysplasia (HED), a condition characterized by sparse hair (oligotrichosis), missing teeth (oligodontia), and reduced ability to sweat (oligohidrosis or anhidrosis) .
Recombinant EDAR (Human, Sf9) refers to the human EDAR protein produced using the Sf9 insect cell expression system. This system is commonly used for producing recombinant proteins due to its high yield and proper post-translational modifications. The recombinant EDAR is utilized in various research applications to study the EDA/EDAR signaling pathway and its implications in development and disease.
The EDA/EDAR signaling pathway has been implicated in several diseases beyond ectodermal dysplasia. Elevated levels of EDA have been observed in metabolic diseases such as non-alcoholic fatty liver disease (NAFLD), obesity, and insulin resistance . Additionally, EDA and its receptors play a role in tumor pathogenesis by regulating tumor cell proliferation, apoptosis, differentiation, and migration .
