EDA2R Human

Ectodysplasin A2 Receptor Human Recombinant
Cat. No.
BT907
Source
Escherichia Coli.
Synonyms
Ectodysplasin A2 Receptor, XEDAR, TNFRSF27, EDA-A2 Receptor, X-Linked Ectodysplasin-A2 Receptor, EDAA2R, EDA-A2R, Tumor Necrosis Factor Receptor Superfamily Member 27, Tumor Necrosis Factor Receptor Superfamily Member XEDAR, EDAR2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EDA2R Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 161 amino acids (1-138 a.a) and having a molecular mass of 17.7kDa.
EDA2R is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The EDA2R protein, also known as ectodysplasin A2 receptor, plays a crucial role in activating the NF-kappa-B and JNK pathways. This activation is believed to occur through its interaction with TRAF3 and TRAF6. Mutations in the EDA gene can lead to a clinical syndrome characterized by the absence or underdevelopment of hair, sweat glands, and teeth. EDA2R exhibits specific binding affinity for the EDA-A2 isoform. Classified as a type III transmembrane protein within the tumor necrosis factor receptor (TNFR) superfamily, EDA2R possesses three cysteine-rich repeats and a single transmembrane domain. Notably, it lacks an N-terminal signal peptide. The gene encoding EDA2R undergoes alternative splicing, resulting in multiple transcript variants. EDA2R has been implicated in several diseases, including X-linked hypohidrotic ectodermal dysplasia.
Description
Recombinant EDA2R, of human origin, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain comprising 161 amino acids (with a sequence spanning from amino acid position 1 to 138) and has a molecular weight of 17.7 kDa. The EDA2R protein is expressed with a 23 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
The EDA2R protein is supplied as a solution at a concentration of 1 mg/ml. The solution also contains 20 mM Tris-HCl buffer with a pH of 8.0, 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, adding a carrier protein (either HSA or BSA) to a final concentration of 0.1% is advisable. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of EDA2R is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Ectodysplasin A2 Receptor, XEDAR, TNFRSF27, EDA-A2 Receptor, X-Linked Ectodysplasin-A2 Receptor, EDAA2R, EDA-A2R, Tumor Necrosis Factor Receptor Superfamily Member 27, Tumor Necrosis Factor Receptor Superfamily Member XEDAR, EDAR2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDCQENE YWDQWGRCVT CQRCGPGQEL SKDCGYGEGG DAYCTACPPR RYKSSWGHHR CQSCITCAVI NRVQKVNCTA TSNAVCGDCL PRFYRKTRIG GLQDQECIPC TKQTPTSEVQ CAFQLSLVEA DAPTVPPQEA T

Product Science Overview

Introduction

The Ectodysplasin A2 Receptor (EDA2R), also known as Tumor Necrosis Factor Receptor Superfamily Member 27 (TNFRSF27) or X-linked Ectodysplasin-A2 Receptor (XEDAR), is a protein encoded by the EDA2R gene in humans . This receptor is part of the tumor necrosis factor (TNF) receptor superfamily and plays a crucial role in the development and maintenance of ectodermal tissues, including hair, teeth, and sweat glands .

Gene and Protein Structure

The EDA2R gene is located on the X chromosome (Xq12) and encodes a type III transmembrane protein . The protein consists of three cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide . The EDA2R protein specifically binds to the EDA-A2 isoform of ectodysplasin, which is a member of the TNF family of ligands .

Function and Signaling Pathways

EDA2R mediates the activation of several signaling pathways, including the NF-kappa-B and JNK pathways . Activation of these pathways is essential for the proper development of ectodermal tissues. The receptor’s interaction with the EDA-A2 isoform is crucial for the maintenance of hair and teeth . Additionally, EDA2R is involved in the positive regulation of the JNK cascade, NF-kappaB transcription factor activity, and intrinsic apoptotic signaling pathways .

Clinical Significance

Mutations in the EDA2R gene can lead to ectodermal dysplasia, a group of disorders characterized by abnormalities in the development of ectodermal tissues . These disorders often result in symptoms such as loss of hair, sweat glands, and teeth . Understanding the function and signaling mechanisms of EDA2R is crucial for developing potential therapeutic strategies for these conditions.

Recombinant EDA2R

Recombinant EDA2R is a human-made version of the natural receptor, produced using recombinant DNA technology. This technology allows for the production of large quantities of the receptor for research and therapeutic purposes. Recombinant EDA2R is used in various studies to understand its role in ectodermal development and to develop potential treatments for ectodermal dysplasia and other related disorders .

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