EDA2R Human, Sf9
Sf9, Baculovirus cells.
Tumor necrosis factor receptor superfamily member 27, X-linked ectodysplasin-A2 receptor, EDA-A2 receptor, Ectodysplasin A2 Receptor, X-Linked Ectodysplasin-A2 Receptor, EDA-A2 Receptor, TNFRSF27, XEDAR, Tumor Necrosis Factor Receptor Superfamily Member XEDAR, Tumor Necrosis Factor Receptor Superfamily Member 27, Ectodysplasin A2 Isoform Receptor, EDA-A2R, EDAA2R.
Description
EDA2R Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 380 amino acids (1-138a.a.) and having a molecular mass of 42.5kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). EDA2R is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The ectodysplasin A2 receptor (EDA2R) plays a crucial role in activating the NF-kappa-B and JNK signaling pathways. This activation is believed to occur through its interaction with TRAF3 and TRAF6 proteins. Mutations in the EDA gene can lead to a clinical condition characterized by the absence of hair, sweat glands, and teeth. EDA2R specifically binds to the EDA-A2 isoform. It belongs to the tumor necrosis factor receptor (TNFR) superfamily and is a type III transmembrane protein. EDA2R comprises three cysteine-rich repeats and a single transmembrane domain, but it lacks an N-terminal signal peptide. Several alternatively spliced transcript variants of this gene have been identified. EDA2R is associated with diseases such as ectodermal dysplasia 1, X-linked hypohidrotic ectodermal dysplasia, and hypohidrotic ectodermal dysplasia.
Recombinant human EDA2R, produced in Sf9 insect cells (Baculovirus expression system), is a single, glycosylated polypeptide chain. It consists of 380 amino acids (1-138a.a.), including a 242 amino acid hIgG-His tag at the C-terminus, and has a molecular mass of 42.5 kDa. However, its apparent molecular size on SDS-PAGE is approximately 40-57 kDa. The protein is purified using proprietary chromatographic techniques.
The EDA2R protein solution is provided at a concentration of 0.5 mg/ml in Phosphate Buffered Saline (pH 7.4) containing 20% glycerol and 1 mM DTT.
Tumor necrosis factor receptor superfamily member 27, X-linked ectodysplasin-A2 receptor, EDA-A2 receptor, Ectodysplasin A2 Receptor, X-Linked Ectodysplasin-A2 Receptor, EDA-A2 Receptor, TNFRSF27, XEDAR, Tumor Necrosis Factor Receptor Superfamily Member XEDAR, Tumor Necrosis Factor Receptor Superfamily Member 27, Ectodysplasin A2 Isoform Receptor, EDA-A2R, EDAA2R.
Sf9, Baculovirus cells.
ADPMDCQENE YWDQWGRCVT CQRCGPGQEL SKDCGYGEGG DAYCTACPPR RYKSSWGHHR CQSCITCAVI NRVQKVNCTA TSNAVCGDCL PRFYRKTRIG GLQDQECIPC TKQTPTSEVQ CAFQLSLVEA DAPTVPPQEA TLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH.
Product Science Overview
Ectodysplasin A2 Receptor (EDA2R), also known as Tumor Necrosis Factor Receptor Superfamily Member 27 (TNFRSF27), is a protein encoded by the EDA2R gene in humans. This receptor is a member of the tumor necrosis factor receptor (TNFR) superfamily and plays a crucial role in the development and differentiation of ectodermal tissues, including hair, sweat glands, and teeth .
EDA2R is a type III transmembrane protein characterized by three cysteine-rich repeats and a single transmembrane domain. Unlike other members of the TNFR superfamily, EDA2R lacks an N-terminal signal peptide . The receptor specifically binds to the EDA-A2 isoform of ectodysplasin, a protein encoded by the anhidrotic ectodermal dysplasia (EDA) gene .
Upon binding to its ligand, EDA2R mediates the activation of the NF-kappa-B and JNK pathways. This activation is facilitated through interactions with TRAF3 and TRAF6 . Mutations in the EDA gene can lead to clinical syndromes characterized by the loss of hair, sweat glands, and teeth, such as hypohidrotic ectodermal dysplasia .
The human recombinant EDA2R is produced in Sf9 Baculovirus cells. This recombinant protein is a single, glycosylated polypeptide chain containing 380 amino acids and has a molecular mass of approximately 42.5 kDa . The recombinant EDA2R is expressed with a 242 amino acid hIgG-His tag at the C-terminus and is purified using proprietary chromatographic techniques .
Recombinant EDA2R is used primarily for laboratory research purposes. It is supplied as a sterile filtered clear solution containing phosphate-buffered saline (pH 7.4), 20% glycerol, and 1 mM DTT . The protein should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. For long-term storage, it is recommended to add a carrier protein such as 0.1% HSA or BSA to prevent freeze-thaw cycles .
