NECTIN3 Human
Sf9, Insect cells.
Nectin-3, CDw113, Nectin cell adhesion molecule 3, Poliovirus receptor-related protein 3, CD113, PVRL3, PRR3, NECTIN-3, PVRR3, PPR3.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
NECTIN3 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 355 amino acids (58-404 a.a.) and having a molecular mass of 39.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
NECTIN3 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
NECTIN3, also referred to as Nectin Cell Adhesion Molecule 3, is a member of the nectin family. This protein plays a crucial role in initiating cell-to-cell adhesion, which in turn promotes cell attachment and facilitates the subsequent formation of intercellular junctions based on JAM and cadherin. NECTIN3 triggers the down-regulation of PVR from the cell surface through endocytosis, leading to a decrease in cell movement and proliferation. The activity of Nectin-3 further strengthens the junction through trans-interaction with various molecules.
Produced in Sf9 Insect cells, NECTIN3 is a single, glycosylated polypeptide chain consisting of 355 amino acids (58-404 a.a.) and possessing a molecular mass of 39.1kDa. It's important to note that on SDS-PAGE, the molecular size will appear approximately between 40-57kDa. The protein is expressed with an 8 amino acid His tag at the C-Terminus and purified using proprietary chromatographic techniques.
A colorless solution that has undergone sterile filtration.
The NECTIN3 protein solution is provided at a concentration of 0.25mg/ml and contains Phosphate Buffered Saline (pH 7.4), 20% glycerol, and 1mM DTT.
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, freezing at -20°C is recommended. To further enhance long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing should be avoided.
The purity of the protein is determined to be greater than 90.0% based on SDS-PAGE analysis.
Nectin-3, CDw113, Nectin cell adhesion molecule 3, Poliovirus receptor-related protein 3, CD113, PVRL3, PRR3, NECTIN-3, PVRR3, PPR3.
Sf9, Insect cells.
GPIIVEPHVT AVWGKNVSLK CLIEVNETIT QISWEKIHGK SSQTVAVHHP QYGFSVQGEY QGRVLFKNYS LNDATITLHN IGFSDSGKYI CKAVTFPLGN AQSSTTVTVL VEPTVSLIKG PDSLIDGGNE TVAAICIAAT GKPVAHIDWE GDLGEMESTT TSFPNETATI ISQYKLFPTR FARGRRITCV VKHPALEKDI RYSFILDIQY APEVSVTGYD GNWFVGRKGV NLKCNADANP PPFKSVWSRL DGQWPDGLLA SDNTLHFVHP LTFNYSGVYI CKVTNSLGQR SDQKVIYISD PPTTTTLQPT IQWHPSTADI EDLATEPKKL PFPLSTLATI KDDTIATLEH HHHHH.
Product Science Overview
Nectin Cell Adhesion Molecule 3, also known as Nectin-3 or NECTIN3, is a protein encoded by the NECTIN3 gene in humans. It belongs to the family of immunoglobulin-like cellular adhesion molecules that are involved in calcium-independent cellular adhesion in various tissues during development . Nectin-3 plays a crucial role in the formation and maintenance of cell-cell junctions, particularly in the nervous system and epithelial tissues.
Nectin-3 has three splicing variants: nectin-3α, nectin-3β, and nectin-3γ. The largest variant, nectin-3α, is abundantly expressed in the testis and is also found in the heart, brain, liver, and kidney . Nectin-3 is characterized by its immunoglobulin-like domains, which facilitate its role in cell adhesion.
Nectin-3 functions as a homophilic cell adhesion molecule, meaning it can bind to itself to mediate cell-cell adhesion. It is also involved in heterophilic interactions with other nectins, such as nectin-1 and nectin-2. These interactions are crucial for the formation of adherens junctions, which are essential for maintaining the structural integrity of tissues .
In the nervous system, nectin-3 is involved in synapse formation and maintenance. It has been shown to play a role in the development of cortical circuits by regulating dendritic spine densities, which are indicative of synaptic connections . Proper levels of nectin-3 are required for normal synapse formation, and both overexpression and knockdown of nectin-3 can lead to abnormalities in synaptic density .
Mutations or dysregulation of nectin-3 can lead to various developmental disorders and diseases. For example, alterations in nectin-3 expression have been linked to intellectual disabilities and other neurodevelopmental disorders. Additionally, nectin-3 is involved in the entry of certain viruses, such as herpes simplex virus, into host cells .
Human recombinant nectin-3 is produced using recombinant DNA technology. This involves inserting the NECTIN3 gene into an expression vector, which is then introduced into a host cell line, such as E. coli or CHO cells. The host cells express the nectin-3 protein, which is subsequently purified using various chromatographic techniques. Recombinant nectin-3 is used in research to study its role in cell adhesion and synapse formation, as well as in the development of therapeutic interventions for diseases associated with nectin-3 dysfunction.
