CDH2 Human
Sf9, Baculovirus cells.
Cadherin 2, Cadherin 2, Type 1, N-Cadherin (Neuronal), Neural Cadherin, N-Cadherin, CDw325, NCAD, CDHN, Calcium-Dependent Adhesion Protein, Neuronal, CD325 Antigen, N-Cadherin 1, Cadherin-2, CD325.
Greater than 90.0% as determined by SDS-PAGE.
Description
CDH2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 574 amino acids (160-724a.a.) and having a molecular mass of 62.9kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
CDH2 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Cadherin-2 isoform 1, also known as CDH2, is a transmembrane glycoprotein involved in cell-to-cell adhesion. It belongs to the calcium-dependent cell adhesion molecule family. Initially found in neurons, CDH2 has also been identified in cardiac muscle and implicated in cancer metastasis. Loss of CDH2 can promote tumor development by releasing membrane-bound β-catenin, which activates Wnt signaling. While CDH2's role in tumorigenesis is not fully understood, it appears to be particularly significant in adrenocortical tumors (ACTs).
Recombinant human CDH2, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein comprises 574 amino acids (residues 160-724), including a C-terminal 6-His tag, and has a molecular weight of 62.9 kDa (appears as 70-100 kDa on SDS-PAGE). Purification is achieved through proprietary chromatographic methods.
The CDH2 protein solution is provided at a concentration of 0.5 mg/ml in Phosphate Buffered Saline (pH 7.4) containing 20% glycerol.
For short-term storage (2-4 weeks), the product can be stored at 4°C. For longer periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Cadherin 2, Cadherin 2, Type 1, N-Cadherin (Neuronal), Neural Cadherin, N-Cadherin, CDw325, NCAD, CDHN, Calcium-Dependent Adhesion Protein, Neuronal, CD325 Antigen, N-Cadherin 1, Cadherin-2, CD325.
Sf9, Baculovirus cells.
ADPDWVIPPI NLPENSRGPF PQELVRIRSD RDKNLSLRYS VTGPGADQPP TGIFIINPIS GQLSVTKPLD REQIARFHLR AHAVDINGNQ VENPIDIVIN VIDMNDNRPE FLHQVWNGTV PEGSKPGTYV MTVTAIDADD PNALNGMLRY RIVSQAPSTP SPNMFTINNE TGDIITVAAG LDREKVQQYT LIIQATDMEG NPTYGLSNTA TAVITVTDVN DNPPEFTAMT FYGEVPENRV DIIVANLTVT DKDQPHTPAW NAVYRISGGD PTGRFAIQTD PNSNDGLVTV VKPIDFETNR MFVLTVAAEN QVPLAKGIQH PPQSTATVSV TVIDVNENPY FAPNPKIIRQ EEGLHAGTML TTFTAQDPDR YMQQNIRYTK LSDPANWLKI DPVNGQITTI AVLDRESPNV KNNIYNATFL ASDNGIPPMS GTGTLQIYLL DINDNAPQVL PQEAETCETP DPNSINITAL DYDIDPNAGP FAFDLPLSPV TIKRNWTITR LNGDFAQLNL KIKFLEAGIY EVPIIITDSG NPPKSNISIL RVKVCQCDSN GDCTDVDRIV GAGLGTGAHH HHHH.
Product Science Overview
Cadherin 2 is a single-pass transmembrane protein that mediates homophilic cell-cell adhesion, meaning it binds to the same type of cadherin on adjacent cells. This adhesion is calcium-dependent, requiring calcium ions to maintain its structural integrity and adhesive function . The protein is composed of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. The extracellular domain contains five cadherin repeats, which are responsible for binding calcium ions and mediating cell-cell adhesion .
Cadherin 2 is expressed in various tissues, including neural tissue, cardiac muscle, and endothelial cells. It plays a pivotal role in several physiological processes:
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Neural Development: Initially named Neural cadherin, Cadherin 2 is essential for the development and maintenance of the nervous system. It facilitates the formation of synaptic connections and is involved in synaptic plasticity, which is crucial for learning and memory .
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Cardiac Muscle: In cardiac muscle, Cadherin 2 is a key component of adherens junctions at intercalated discs. These junctions mechanically and electrically couple adjacent cardiomyocytes, ensuring coordinated contraction of the heart muscle .
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Cancer Metastasis: Cadherin 2 is also implicated in cancer metastasis. Changes in its expression or function can lead to increased cell motility and invasiveness, contributing to the spread of cancer cells .
Cadherin 2 has significant clinical implications. Its role in cancer metastasis makes it a potential target for cancer therapy. Additionally, mutations in the CDH2 gene, which encodes Cadherin 2, have been linked to various diseases, including arrhythmogenic right ventricular dysplasia (ARVD) and other cardiac disorders .
Recombinant Cadherin 2 (Human) is a form of the protein produced through recombinant DNA technology. This allows for the production of large quantities of the protein for research and therapeutic purposes. Recombinant Cadherin 2 is used in various biomedical research applications, including studies on cell adhesion, cancer metastasis, and cardiac function .
