CDH11 Human

Cadherin 11 Human Recombinant
Cat. No.
BT27688
Source
E.coli.
Synonyms
CAD11, CDHOB, OB, OSF-4, Cadherin 11, Osteoblast cadherin, OB-cadherin.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CDH11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 601 amino acids (54-617 a.a) and having a molecular mass of 66.2kDa. CDH11 is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cadherin 11 (CDH11), a member of the cadherin superfamily, is an integral membrane protein involved in calcium-dependent cell-cell adhesion. Unlike type I cadherins, CDH11 lacks the HAV cell adhesion recognition sequence. Its expression in osteoblastic cell lines and upregulation during differentiation suggest a role in bone development and maintenance. CDH11 also contributes to the sorting of different cell types.
Description
Recombinant human CDH11, produced in E. coli, is a non-glycosylated polypeptide chain with 601 amino acids (residues 54-617). It has a molecular weight of 66.2 kDa. The protein consists of a 37 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless, and clear solution.
Formulation
The CDH11 protein solution has a concentration of 1mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
CAD11, CDHOB, OB, OSF-4, Cadherin 11, Osteoblast cadherin, OB-cadherin.
Source
E.coli.
Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGWV WNQFFVIEEY TGPDPVLVGR LHSDIDSGDG NIKYILSGEG AGTIFVIDDK SGNIHATKTL DREERAQYTL MAQAVDRDTN RPLEPPSEFI VKVQDINDNP PEFLHETYHA NVPERSNVGT SVIQVTASDA DDPTYGNSAK LVYSILEGQP YFSVEAQTGI IRTALPNMDR EAKEEYHVVI QAKDMGGHMG GLSGTTKVMI TLTDVNDNPP KFPQSVYQMS VSEAAVPGEE VGRVKAKDPD IGENGLVTYN IVDGDGMESF EITTDYETQE GVIKLKKPVD FETKRAYSLK VEAANVHIDP KFISNGPFKD TVTVKIAVED ADEPPMFLAP SYIHEVQENA AAGTVVGRVH AKDPDAANSP IRYSIDRHTD LDRFFTINPE DGFIKTTKPL DREETAWLNI TVFAAEIHNR HQEAKVPVAI RVLDVNDNAP KFAAPYEGFI CESDQTKPLS NQPIVTISAD DKDDTANGPR FIFSLPPEII HNPNFTVRDN RDNTAGVYAR RGGFSRQKQD LYLLPIVISD GGIPPMSSTN TLTIKVCGCD VNGALLSCNA EAYILNAGLS T.

Product Science Overview

Structure and Function

Cadherin-11 is a type II classical cadherin, characterized by a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain . The protein mediates calcium-dependent cell-cell adhesion, which is essential for maintaining the structural integrity of tissues .

Expression and Role in Development

Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems . In adults, it is predominantly expressed on osteoblasts, where it promotes the differentiation of both osteoblasts and chondrocytes . This makes it a critical player in bone development and maintenance.

Clinical Significance

Cadherin-11 is up-regulated in certain types of cancer, including breast and prostate cancers, which preferentially metastasize to bone . Its expression is also increased in fibrotic diseases such as systemic sclerosis and chronic inflammatory arthritis . These associations make Cadherin-11 a potential target for therapeutic interventions in these conditions.

Recombinant Human Cadherin-11

Recombinant Human Cadherin-11 is produced using various expression systems, including NS0 mouse myeloma cells and HEK293 cells . The recombinant protein is typically purified to high levels of purity and is used in various research applications, including studies on cell adhesion, cancer metastasis, and bone development.

The recombinant form of Cadherin-11 is often tagged with polyhistidine or other tags to facilitate purification and detection . It is available in lyophilized form and can be reconstituted in sterile buffers for use in laboratory experiments .

Applications

Recombinant Human Cadherin-11 is used in a variety of research applications, including:

  • Cell adhesion studies: To understand the mechanisms of cell-cell adhesion and the role of cadherins in maintaining tissue integrity.
  • Cancer research: To study the role of Cadherin-11 in cancer metastasis, particularly in bone metastasis of breast and prostate cancers.
  • Bone development: To investigate the role of Cadherin-11 in osteoblast and chondrocyte differentiation and bone formation.

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