CDH5 Human

Cadherin 5 Human Recombinant
Cat. No.
BT27844
Source
Sf9, Insect cells.
Synonyms
Cadherin 5, VE-Cadherin, Cadherin 5 Type 2 VE-Cadherin (Vascular Epithelium), CDH5, Cadherin 5 Type 2 (Vascular Endothelium), Vascular Endothelial Cadherin, 7B4 Antigen, Endothelial-Specific Cadherin, Cd144 Antigen, CD144 Antigen, Cadherin-5, CD144, 7B4.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Cadherin 5 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 810 amino acids (29-599 a.a.) and having a molecular mass of 91.5kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). CDH5 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cadherin-5 (CDH5), a member of the atypical/type 2 subgroup of Cadherin homophilic adhesion proteins, is a calcium-dependent cell-cell adhesion molecule. It comprises five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. This protein plays a crucial role in the formation, maturation, and remodeling of the vascular wall. CDH5 is primarily found in vascular endothelia, where it acts as either the sole or predominant cadherin, often coexisting with N-cadherin. It regulates and is regulated by various factors, including VEGF R2, type 1 and type 2 TGF-beta receptors, and endothelial junction proteins like JAM-C, Claudin-5, and N-Cadherin. Additionally, CDH5 interacts with alpha-catenin, establishing a link to the cytoskeleton. It collaborates with KRIT1 to establish and maintain endothelial cell polarity and vascular lumen integrity. Furthermore, CDH5 is vital for activating PRKCZ and localizing phosphorylated PRKCZ, PARD3, TIAM1, and RAP1B to the cell junction. The CDH5 gene is situated within a gene cluster on chromosome 16's long arm, a region associated with loss of heterozygosity events in breast and prostate cancer.
Description
Recombinant Human Cadherin 5, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with 810 amino acids (29-599 a.a.). It has a molecular mass of 91.5kDa and migrates at 70-100kDa on SDS-PAGE under reducing conditions. This CDH5 protein is expressed with a C-terminal 239 amino acid hIgG-His-tag and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The CDH5 solution is provided at a concentration of 0.5mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep the vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It's crucial to avoid repeated freeze-thaw cycles.
Purity
The purity of CDH5 is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Cadherin 5, VE-Cadherin, Cadherin 5 Type 2 VE-Cadherin (Vascular Epithelium), CDH5, Cadherin 5 Type 2 (Vascular Endothelium), Vascular Endothelial Cadherin, 7B4 Antigen, Endothelial-Specific Cadherin, Cd144 Antigen, CD144 Antigen, Cadherin-5, CD144, 7B4.
Source
Sf9, Insect cells.
Amino Acid Sequence
NPAQRDTHSL LPTHRRQKRD WIWNQMHIDE EKNTSLPHHV GKIKSSVSRK NAKYLLKGEY VGKVFRVDAE TGDVFAIERL DRENISEYHL TAVIVDKDTG ENLETPSSFT IKVHDVNDNW PVFTHRLFNA SVPESSAVGT SVISVTAVDA DDPTVGDHAS VMYQILKGKE YFAIDNSGRI ITITKSLDRE KQARYEIVVE ARDAQGLRGD SGTATVLVTL QDINDNFPFF TQTKYTFVVP EDTRVGTSVG SLFVEDPDEP QNRMTKYSIL RGDYQDAFTI ETNPAHNEGI IKPMKPLDYE YIQQYSFIVE ATDPTIDLRY MSPPAGNRAQ VIINITDVDE PPIFQQPFYH FQLKENQKKP LIGTVLAMDP DAARHSIGYS IRRTSDKGQF FRVTKKGDIY NEKELDREVY PWYNLTVEAK ELDSTGTPTG KESIVQVHIE VLDENDNAPE FAKPYQPKVC ENAVHGQLVL QISAIDKDIT PRNVKFKFIL NTENNFTLTD NHDNTANITV KYGQFDREHT KVHFLPVVIS DNGMPSRTGT STLTVAVCKC NEQGEFTFCE DMAAQVGVSI QLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH.

Product Science Overview

Structure and Function

Cadherin 5 is a single-pass transmembrane protein that mediates calcium-dependent cell-cell adhesion. It has five extracellular cadherin domains that facilitate homophilic interactions between adjacent endothelial cells . These interactions are vital for the formation of adherens junctions, which are necessary for the stability and permeability of blood vessels.

The protein is encoded by the CDH5 gene, which is located on chromosome 16 in humans . The gene is highly conserved across species, indicating its essential role in vascular biology.

Biological Significance

Cadherin 5 is involved in various biological processes, including:

  • Vascular Development: It is crucial for the formation of new blood vessels (angiogenesis) and the maintenance of existing ones.
  • Endothelial Cell Function: It regulates endothelial cell behavior, including cell migration, proliferation, and survival.
  • Barrier Function: It maintains the integrity of the endothelial barrier, preventing the leakage of blood components into surrounding tissues.
Clinical Relevance

Cadherin 5 has been studied extensively for its role in various diseases, particularly cancer. It has been identified as a biomarker for metastatic breast cancer, especially in estrogen receptor-positive cancers with vascular invasion . Elevated levels of Cadherin 5 and its glycosylation status have been associated with poor prognosis in breast cancer patients .

Additionally, mutations or dysregulation of the CDH5 gene can lead to vascular defects and contribute to the development of various cardiovascular diseases .

Recombinant Cadherin 5

Recombinant Cadherin 5 (Human) is produced using recombinant DNA technology, which involves inserting the human CDH5 gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of the protein for research and therapeutic purposes.

Recombinant Cadherin 5 is used in various applications, including:

  • Research: It is used to study the molecular mechanisms of endothelial cell adhesion and signaling.
  • Drug Development: It serves as a target for developing therapies aimed at modulating vascular function and treating diseases such as cancer and cardiovascular disorders.
  • Diagnostics: It is used in diagnostic assays to detect and quantify Cadherin 5 levels in biological samples.

In conclusion, Cadherin 5 (Human Recombinant) is a vital protein with significant roles in vascular biology and disease. Its recombinant form provides valuable tools for research, diagnostics, and therapeutic development.

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