POU5F1 Human, PolyR
POU Class 5 Homeobox 1 Human Recombinant, Polyarginine-Tag
Cat. No.
BT5488
Source
Escherichia Coli.
Synonyms
POU domain class 5 transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3, OTF4, MGC22487.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
POU5F1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 360 amino acids and having a molecular mass of 38.6kDa.
The POU5F1 is fused to a C-terminal poly-arginine tag and purified by proprietary chromatographic techniques.
The POU5F1 is fused to a C-terminal poly-arginine tag and purified by proprietary chromatographic techniques.
Product Specs
Introduction
POU5F1, a homeodomain transcription factor belonging to the POU family, is primarily expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells. This factor plays a critical role in the signaling pathway responsible for maintaining the self-renewal and pluripotency of ES cells. POU5F1 possesses two distinct DNA binding domains that independently bind to half-sites of the canonical octamer motif. This flexibility enables POU5F1 to interact with various DNA motifs by forming heterodimers with other transcription factors or homodimers in different conformations. Human POU5F1 comprises a 75 amino acid POU specific (POUS) domain and a 60 amino acid POU-Homeo- (POUH) domain, connected by a linker region. Human POU5F1 exhibits specific binding affinity for the Octamer motif ATGCAAAT. Additionally, two proline-rich domains located in the N-terminal and C-terminal regions are essential for POU5F1 transactivation. POU5F1 regulates numerous target genes and has been shown to collaborate with other transcription factors, including Sox2 and Nanog, to maintain stem cell potency and self-renewal.
Description
Recombinant Human POU5F1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 360 amino acids. It has a molecular weight of 38.6 kDa. The POU5F1 protein is fused to a C-terminal poly-arginine tag and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
POU5F1 is supplied as a 0.2 µm filtered solution in PBS at a pH of 7.4 with 5% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. It is important to avoid repeated cycles of freezing and thawing.
Purity
The purity is greater than 95.0% as determined by: (a) Size Exclusion Chromatography-High Performance Liquid Chromatography (SEC-HPLC) analysis. (b) Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Synonyms
POU domain class 5 transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3, OTF4, MGC22487.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Gly-His-Leu.
Product Science Overview
Structure and Domains
POU5F1 contains two distinct DNA-binding domains:
- POU-specific (POUS) domain: This domain consists of 75 amino acids.
- POU-homeo (POUH) domain: This domain consists of 60 amino acids.
These domains are connected by a linker region, allowing POU5F1 to bind to the canonical octamer motif (ATGCAAAT) either as a homodimer or heterodimer with other transcription factors .
Function and Mechanism
POU5F1 is essential for the transcriptional regulation of genes involved in maintaining stem cell potency and self-renewal. It interacts with other transcription factors such as Sox2 and Nanog to regulate target genes . The protein has two proline-rich domains in the N-terminal and C-terminal regions, which are vital for its transactivation function .
Recombinant POU5F1 with Polyarginine-Tag
The recombinant version of POU5F1 is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 360 amino acids. It has a molecular mass of approximately 38.6 kDa and is fused to a C-terminal polyarginine tag . This tag aids in the purification and detection of the protein.
Applications
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