POU5F1 Human

POU Class 5 Homeobox 1 Human Recombinant
Cat. No.
BT5407
Source
Escherichia Coli.
Synonyms
POU domain class 5 transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3, OTF4, MGC22487.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POU5F1 Human Recombinant produced in E. coli is a single polypeptide chain containing 285 amino acids (1-265) and having a molecular mass of 32.2 kDa.
POU5F1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
POU5F1, a member of the POU family, is a homeodomain transcription factor found in embryonic stem (ES) and embryonic carcinoma (EC) cells. It plays a critical role in maintaining the self-renewal and pluripotency of ES cells through its involvement in the signaling pathway. The protein possesses two separate DNA binding domains that bind to half-sites of the canonical octamer motif independently. This characteristic allows POU5F1 to interact with various DNA motifs, either by forming heterodimers with other transcription factors or homodimers in multiple conformations. Human POU5F1 consists of a 75-amino acid POU-specific (POUS) domain and a 60-amino acid POU-Homeo- (POUH) domain linked by a linker region. It specifically interacts with the Octamer motif ATGCAAAT. Moreover, two proline-rich domains located in the N-terminal and C-terminal regions are crucial for POU5F1 transactivation. POU5F1 regulates numerous target genes and collaborates with other transcription factors, such as Sox2 and Nanog, to maintain stem cell potency and self-renewal.
Description
Recombinant Human POU5F1, produced in E. coli, is a single polypeptide chain consisting of 285 amino acids (1-265) with a molecular weight of 32.2 kDa. It features a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The POU5F1 solution is formulated in 20mM Tris-HCl buffer with a pH of 8.0, 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the entire vial can be stored at 4°C. For extended periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
POU domain class 5 transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3, OTF4, MGC22487.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MHFYRLFLGA TRRFLNPEWK GEIDNWCVYV LTSLLPFKIQ SQDIKALQKE LEQFAKLLKQ KRITLGYTQA DVGLTLGVLF GKVFSQTTIC RFEALQLSFK NMCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN.

Product Science Overview

Structure and Function

POU5F1 contains a POU homeodomain, which allows it to bind to specific DNA sequences and regulate the expression of target genes . It forms a trimeric complex with other transcription factors such as SOX2 or SOX15 on DNA, controlling the expression of genes involved in early embryonic development, including YES1, FGF4, UTF1, and ZFP206 .

Role in Embryonic Development

POU5F1 is essential for the maintenance of pluripotency in embryonic stem cells (ESCs) and germline cells . It was first identified in mice as an ESC-specific and germline-specific transcription factor . The protein’s ability to maintain pluripotency makes it a key player in early embryonic development, ensuring that cells retain the potential to differentiate into various cell types.

Clinical Significance

Aberrant expression of POU5F1 in adult tissues is associated with tumorigenesis . For instance, it can participate in a translocation with the Ewing’s sarcoma gene on chromosome 21, leading to tumor formation . Additionally, diseases such as Embryonal Carcinoma and Premature Ovarian Failure 5 have been linked to mutations or dysregulation of the POU5F1 gene .

Recombinant POU5F1

Human recombinant POU5F1 is produced using recombinant DNA technology, which involves inserting the human POU5F1 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in various research applications, including studies on stem cell biology, cancer research, and developmental biology.

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THE BIOTEK
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