POU6F1 Human
Description
POU6F1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
POU Class 6 Homeobox 1, also known as POU6F1, is a protein-coding gene that plays a crucial role in the regulation of gene expression. This protein belongs to the POU family of transcription factors, which are characterized by the presence of a specific DNA-binding domain known as the POU domain. The POU domain enables these proteins to bind to specific DNA sequences and regulate the transcription of target genes.
The POU6F1 gene is located on chromosome 12q13.13 and spans approximately 31 kilobases. It encodes a protein that is 301 amino acids in length and has an apparent molecular weight of approximately 50 kDa . The gene is expressed in various tissues, including the brain, skeletal muscle, heart, and lung . In the brain, POU6F1 expression is particularly high in specific cell layers of the cortex, olfactory bulb, hippocampus, and cerebellum .
POU6F1 functions as a transcription factor by binding to octamer or octamer-related motifs in the DNA via its POU domain . This binding regulates the lineage-specific expression of various genes. For instance, POU6F1 has been shown to bind to multiple motifs in the TCR-beta enhancer, suggesting its role in regulating the expression of the TCR-beta gene . Additionally, POU6F1 can bind to immunoglobulin octamer and octamer-like motifs, indicating its potential role in regulating other lymphoid genes .
Recombinant POU6F1 is a laboratory-produced version of the human POU6F1 protein. It is typically expressed in bacterial or mammalian cell systems and purified for use in various research applications. Recombinant POU6F1 is used to study the protein’s function, interactions, and regulatory mechanisms. It is also employed in assays to investigate the binding of POU6F1 to specific DNA sequences and its role in gene transcription.
The study of POU6F1 and its recombinant form has significant implications in understanding gene regulation and transcriptional control. Researchers use recombinant POU6F1 to explore its role in various biological processes, including development, differentiation, and immune response. Additionally, POU6F1 is of interest in the study of neurological disorders, given its high expression in the brain and its involvement in regulating genes critical for brain function.
