POU2AF1 Human

POU class 2 associating factor 1 Human Recombinant
Cat. No.
BT5334
Source
Escherichia Coli.
Synonyms
POU domain class 2 associating factor 1, BOB-1, OCA-B, B-cell-specific coactivator OBF-1, OCT-binding factor 1.
Appearance
POU2AF1 is supplied as a sterile filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POU2AF1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 276 amino acids (1-256) and having a molecular mass of 29.6 kDa.
The POU2AF1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
POU2AF1, a lymphocyte-specific transcription coactivator protein, enhances the transcriptional efficiency of Oct1/2 proteins by interacting with their POU domains. While lacking inherent DNA binding ability, POU2AF1 firmly binds to the octomer motif in the presence of Oct1 and Oct2. Its expression is highest in spleen and peripheral blood leukocytes.
Description
Recombinant human POU2AF1, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 276 amino acids (residues 1-256). With a molecular weight of 29.6 kDa, it features a 20-amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
POU2AF1 is provided as a clear, sterile solution after filtration.
Formulation
The POU2AF1 protein solution (0.5 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8) and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
SDS-PAGE analysis indicates a purity exceeding 85%.
Synonyms
POU domain class 2 associating factor 1, BOB-1, OCA-B, B-cell-specific coactivator OBF-1, OCT-binding factor 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT LSVEGF

Product Science Overview

Introduction

POU Class 2 Associating Factor 1 (POU2AF1), also known as Oct coactivator from B cells (OCAB), Oct binding factor 1 (OBF1 or OBF-1), and BOB1, is a protein encoded by the POU2AF1 gene in humans . This protein plays a crucial role in the regulation of gene expression, particularly in B cells, and is involved in various biological processes, including immune response and transcription regulation.

Gene and Protein Structure

The POU2AF1 gene is located on chromosome 11 and encodes a protein that is approximately 256 amino acids in length . The protein contains a POU-specific domain, which is essential for its interaction with other transcription factors such as POU2F1 (OCT1) and POU2F2 (OCT2) . These interactions are critical for the transcriptional activation of target genes.

Function and Mechanism of Action

POU2AF1 functions as a transcriptional coactivator, meaning it enhances the transcriptional activity of other transcription factors. It specifically associates with POU2F1/OCT1 and POU2F2/OCT2, boosting their promoter activity . This interaction is vital for the proper functioning of B cells, particularly in their response to antigens and the formation of germinal centers .

In B cells, POU2AF1 regulates the expression of interleukin-6 (IL-6), a cytokine involved in immune responses . The protein’s role as a coactivator is essential for the transcriptional regulation of genes involved in immune function and development.

Clinical Significance

Mutations or dysregulation of the POU2AF1 gene have been associated with various diseases, including primary biliary cholangitis and lateral myocardial infarction . Additionally, POU2AF1 has been identified as a hub gene in osteoporosis (OP) progression, where it plays a role in osteoblast apoptosis . Overexpression of POU2AF1 has been shown to decrease the expression of cleaved caspase-3/-8/-9, which are markers of apoptosis, while increasing the expression of cyclinD1 and Ki67, which are markers of cell proliferation .

Applications in Research and Medicine

Human recombinant POU2AF1 is used in research to study its role in transcription regulation and immune response. It is also utilized in various assays to investigate its function in different cellular processes and disease models. Understanding the mechanisms by which POU2AF1 regulates gene expression can provide insights into potential therapeutic targets for diseases associated with its dysregulation.

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