Glycinin
Escherichia Coli.
Glycinin, Arah3.
Greater than 95% as determined by SDS-PAGE.
Description
Recombinant Glycinin produced in E. coli is a non- glycosylated, polypeptide chain having a calculated molecular mass of 63 kDa.
Glycinin is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Glycinin, Arah3.
Escherichia Coli.
Product Science Overview
Allergen Ara h 3.0101, also known as Glycinin, is a major peanut allergen belonging to the legumin family of proteins. It is a storage protein found in peanuts and is known for its ability to withstand heat treatment and enzymatic activity. This allergen is significant due to its role in peanut allergies, which affect a substantial portion of the population, particularly in Western countries.
Ara h 3 is a cupin allergen, specifically an 11S globulin, which is a hexameric protein present in the seeds of many plants . The protein provides a significant portion of the total protein content in peanuts, along with other major allergens such as Ara h 1 and Ara h 2 . The recombinant form of Ara h 3 is produced using molecular cloning techniques, which allow for the generation of well-characterized, non-contaminated allergen components .
Ara h 3 is a major allergen for individuals with peanut allergies. Studies have shown that a significant percentage of peanut-allergic patients are sensitized to Ara h 3, and this sensitization is associated with more severe allergic reactions, including anaphylaxis . The use of specific IgE to Ara h 3, in combination with Ara h 1 and Ara h 2, can be helpful in diagnosing peanut allergies .
Peanut allergies are prevalent in Western nations, with estimated rates between 1-2% . The prevalence appears to be lower in Asia and other global regions, although comprehensive epidemiological studies are limited . Sensitization to Ara h 3 is particularly significant in children, with the highest frequency of positive test results observed in the three to nine-year-old age group .
The development of recombinant allergens, including Ara h 3, has opened new avenues for allergy diagnosis and research. Recombinant allergens are widely used in molecule-based allergy diagnosis, such as protein microarrays or suspension arrays . These technologies have enabled the production of well-characterized vaccine components with known biological activities, including hypoallergenic derivatives with reduced IgE reactivity . Recombinant allergens have been successfully used in immunotherapy trials to treat various allergies, including birch and grass pollen allergies .
