Ara h 2.0201

Allergen Ara h 2.0201, also known as Conglutin-7, is a significant peanut allergen. It is one of the primary proteins responsible for peanut allergies, which can trigger severe allergic reactions in sensitive individuals. This protein is a member of the 2S albumin family and is known for its ability to bind Immunoglobulin E (IgE) antibodies, which play a crucial role in allergic responses.

Ara h 2.0201 is a glycosylated polypeptide chain with a calculated molecular mass of approximately 20,000 Daltons . It is produced recombinantly in Sf9 insect cells and is expressed with a 10xHis tag at the N-terminus, which facilitates its purification through chromatographic techniques . The protein is supplied in a sterile, filtered clear solution and is formulated in 20mM HEPES buffer at pH 7.9 with 6M Urea .

Ara h 2.0201 is a weak inhibitor of trypsin and serves as a conglutin storage protein. Along with Ara h 6.0101, it accounts for the majority of the IgE immune response in individuals with peanut allergies . The protein’s ability to bind IgE antibodies makes it a potent elicitor of allergic reactions, including anaphylaxis . This binding is a critical factor in the diagnosis and study of peanut allergies.

Recombinant Ara h 2.0201 is produced using advanced biotechnological methods. The protein is expressed in Sf9 insect cells, which allows for proper glycosylation and folding, ensuring its biological activity and stability . The recombinant protein is purified to a high degree, with a purity greater than 95% as determined by SDS-PAGE . This high level of purity is essential for its use in research and diagnostic applications.

The recombinant form of Ara h 2.0201 is primarily used in laboratory research to study peanut allergies and to develop diagnostic tests. It is utilized in immunodot tests with positive and negative sera panels to detect the presence of IgE antibodies specific to this allergen . Additionally, it serves as a valuable tool in the development of therapeutic interventions aimed at desensitizing individuals to peanut allergens.

For optimal stability, Ara h 2.0201 should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it should be kept frozen at -20°C. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity and functionality .