Conglutin
Conglutin, Allergen Ara h 6.
Description
Recombinant Conglutin produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 16,217 Dalton.
Conglutin is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Conglutin, also referred to as Ara h 6.0101, belongs to the 2S albumin protein family. It is recognized as one of the most potent elicitors of anaphylaxis, alongside Ara h 2.0201. Both proteins possess multiple disulfide-bridged cysteine residues, contributing to a tightly coiled structure that exhibits high stability against heat and protease degradation. Conglutin can trigger allergic reactions in humans and demonstrate the ability to bind to IgE antibodies.
Recombinant Conglutin, expressed in SF9 insect cells, is a glycosylated polypeptide chain with a calculated molecular mass of 16,217 Daltons.
The recombinant Conglutin is engineered with a 10xHis tag at the N-terminus to facilitate purification, which is carried out using proprietary chromatographic methods.
Conglutin is supplied in a buffer solution containing 20mM HEPES at pH 7.9 and 6M Urea.
2. Demonstrates reactivity in immunodot assays when tested against panels of positive and negative sera.
Conglutin, Allergen Ara h 6.
Product Science Overview
Allergen Ara h 6.0101, also known as Conglutin, is a protein belonging to the 2S albumin family. This protein is derived from peanuts (Arachis hypogaea) and is recognized as one of the most potent allergens responsible for peanut-induced allergic reactions. Ara h 6.0101, along with Ara h 2.0201, is known for its ability to elicit severe anaphylactic reactions in sensitized individuals .
Ara h 6.0101 is a seed storage protein that plays a crucial role in providing amino acids for the growing seedlings and in pathogen defense. It is characterized by its tightly coiled, heat-stable, and protease-resistant core structure, which is maintained by multiple disulfide-bridged cysteine residues . This structural stability allows Ara h 6.0101 to withstand the harsh conditions of the gastrointestinal tract, making it a potent allergen .
Peanut allergy is a significant health concern, particularly in Western nations, where prevalence rates range between 1-2% . Ara h 6.0101 is considered one of the major peanut allergens, alongside Ara h 2. Sensitization to Ara h 6.0101 is associated with severe allergic reactions, including anaphylaxis. Individuals sensitized to Ara h 6.0101 often exhibit cross-sensitivity to other peanut allergens, such as Ara h 2 .
Recombinant Ara h 6.0101 is produced using advanced biotechnological methods. The protein is typically expressed in Sf9 insect cells and purified using proprietary chromatographic techniques. The recombinant form of Ara h 6.0101 is a glycosylated polypeptide chain with a calculated molecular mass of approximately 16.2 kDa . It is supplied in a sterile, filtered solution and formulated in specific buffers to ensure stability and functionality .
