Ara h 8.0101

Allergen Ara h 8.0101 is a recombinant protein derived from the peanut (Arachis hypogaea). It belongs to the PR-10 protein family and is a Bet v 1-homologous panallergen. This protein is known for its role in causing allergic reactions, particularly oral allergy syndrome (OAS), which is a type of food allergy that occurs in individuals sensitized to pollen allergens.

Ara h 8.0101 is a recombinant protein that is typically expressed in microbial systems such as Pichia pastoris or Escherichia coli. The molecular weight of Ara h 8.0101 is approximately 17 kDa . The protein is purified using techniques such as HPLC gel filtration to achieve a purity of over 95% .

Ara h 8.0101 exhibits cross-reactivity with other allergens, particularly those from soybeans (Gly m 4) and birch pollen (Bet v 1) . This cross-reactivity is due to the structural similarities between these proteins, which can lead to allergic reactions in individuals sensitized to any of these allergens.

The stability of Ara h 8.0101 is relatively low when exposed to heat and gastric digestion . This characteristic is significant because it affects the allergen’s ability to cause reactions after being ingested and processed by the digestive system.

Recombinant Ara h 8.0101 is used primarily for research purposes. It is utilized in studies aimed at understanding the mechanisms of allergic reactions and developing diagnostic tools and treatments for peanut allergies . The protein is formulated in preservative-free and carrier-free phosphate-buffered saline (PBS) at pH 7.4 and is stored at -20°C to maintain its stability .