MALD3
Sf9 insect cells.
Non-specific lipid-transfer protein, LTP, MALD3.
Greater than 80.0% as determined by SDS-PAGE.
Description
Recombinant Non-Specific Lipid-Transfer Protein Mal d 3 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 12kDa.
MALD3 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Product Specs
Non-specific lipid-transfer protein, LTP, MALD3.
Sf9 insect cells.
Product Science Overview
Non-Specific Lipid-Transfer Protein Mal d 3 (nsLTP1) is a well-known allergen found in apples (Malus domestica). It is recognized as an important pan-allergen due to its ability to cause allergic reactions in sensitive individuals. The recombinant form of Mal d 3 is used extensively in research and diagnostic applications to study apple allergies and cross-reactivity with other allergens.
Mal d 3 is a small protein with a molecular weight of approximately 9 kDa . It belongs to the family of non-specific lipid-transfer proteins (nsLTPs), which are involved in the transfer of lipids between membranes. This protein is highly stable, resistant to heat, and can withstand pepsin digestion . These properties make it a potent allergen, capable of triggering severe allergic reactions.
Mal d 3 is primarily found in the peel of apples and is known to cause systemic allergic reactions such as diarrhea, vomiting, urticaria, hypotension, asthma, and even anaphylaxis in sensitized individuals . The prevalence of apple allergy due to Mal d 3 sensitization is higher in European countries, particularly in the Mediterranean regions .
Recombinant Mal d 3 is produced using various expression systems, such as E. coli, to obtain a highly pure form of the protein for research and diagnostic purposes . The recombinant protein is often fused with a his-tag at its N-terminus to facilitate purification and detection . It is used in various immunoassays, including Western Blot, Dot Blot, Indirect ELISA, and Chemiluminescent Immunoassay .
Mal d 3 exhibits extensive cross-reactivity with other nsLTP-containing foods and pollen due to high structural homology and sequence identity . This cross-reactivity is observed with members of the Rosaceae family, such as peach, as well as other nsLTPs like mustard (Sin a 3), lettuce (Lac s 1), and raspberry (Rub i 3) .
The prevalence of Mal d 3 sensitization varies across different regions. Studies have shown that sensitization to Mal d 3 is more common in Mediterranean countries of Europe . For instance, a study conducted among apple-allergic patients in Spain demonstrated a high rate of Mal d 3 sensitization . Similarly, other studies have reported significant IgE reactivity to Mal d 3 in apple-allergic individuals .
