Gly m 4.0101

Stress-Induced Protein SAM22 Recombinant
Cat. No.
BT17401
Source
Sf9 insect cells.
Synonyms
Stress-induced protein SAM22, Starvation-associated message 22, Gly m 4.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Recombinant Stress-Induced Protein SAM22 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 19,484 Dalton.
Gly m 4.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Stress-Induced Protein SAM22, also known as Gly m 4.0101, is a soy allergen that primarily affects individuals with birch pollen allergies. It is a significant allergen for those with soy sensitivities.
Description
Recombinant Stress-Induced Protein SAM22, expressed in SF9 cells, is a glycosylated polypeptide chain with a molecular weight of 19,484 Daltons. The protein is tagged with a 10xHis tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
Gly m 4.0101 is provided in a solution containing 20mM HEPES buffer (pH 7.9) and 6M Urea.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For longer storage, it should be stored frozen at -20°C. Repeated freezing and thawing should be avoided.
Purity
The purity of the product is greater than 80.0%, as assessed by SDS-PAGE.
Immunological Functions
This protein exhibits the following immunological properties: 1. It can bind to human IgE antibodies. 2. It demonstrates reactivity in immunodot assays using positive and negative serum panels.
Synonyms
Stress-induced protein SAM22, Starvation-associated message 22, Gly m 4.
Source
Sf9 insect cells.

Product Science Overview

Introduction

Stress-induced proteins play a crucial role in the defense mechanisms of plants. One such protein is the Stress-Induced Protein SAM22, which has been extensively studied in soybeans. This protein is part of the PR-10 (Pathogenesis-Related) protein family and is known for its role in plant stress responses, particularly in relation to biotic and abiotic stress factors.

Discovery and Characterization

The SAM22 protein was first identified in soybeans (Glycine max) and has been the subject of various studies due to its significant role in stress responses. The gene encoding SAM22 was isolated from two different soybean cultivars, Glycine max cv. Mandarin and Glycine max cv. Williams . The mRNAs corresponding to these cDNAs, called SAM22 and H4, respectively, accumulate predominantly in response to stress conditions.

Function and Mechanism

SAM22 is involved in the plant’s defense mechanisms against various stress factors, including pathogen attacks and environmental stresses such as drought and salinity. The protein is upregulated in response to these stress conditions, indicating its role in the plant’s adaptive responses . The mechanism by which SAM22 functions involves the induction of a synthesis of anti-digestive proteins, reactive oxygen species scavenging, signaling pathways, and secondary metabolites synthesis .

Recombinant SAM22

Recombinant SAM22 refers to the protein produced through recombinant DNA technology, which allows for the expression of the SAM22 gene in a host organism, such as bacteria or yeast. This technology enables the production of large quantities of the protein for research and potential agricultural applications. Recombinant SAM22 has been used in various studies to understand its structure, function, and potential applications in enhancing plant resistance to stress.

Applications and Future Prospects

The study of SAM22 and its recombinant form has significant implications for agriculture. By understanding the role of SAM22 in stress responses, researchers can develop strategies to enhance crop resistance to various stress factors, thereby improving crop yield and sustainability. Future research may focus on the genetic engineering of crops to overexpress SAM22, providing them with enhanced resistance to environmental stresses and pathogens.

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