Der F1

The recombinant Der f 1 protein is typically produced using Escherichia coli (E. coli) as the expression system . The gene encoding the Der f 1 protein is cloned into an expression vector, which is then introduced into E. coli cells. These cells are cultured under specific conditions to express the Der f 1 protein, which is subsequently purified using chromatographic techniques to achieve a high level of purity .

Der f 1 is a thiol protease, belonging to the C1 peptidase family . It hydrolyzes proteins, showing a preference for phenylalanine (Phe) or basic residues. The protein has extensive endopeptidase specificity, meaning it can cleave peptide bonds within a protein chain .

Der f 1 is a major allergen found in house dust mite feces. It is known to trigger allergic reactions in sensitive individuals. The protein’s allergenic properties are due to its ability to break down proteins in the human body, leading to an immune response . Common symptoms of mite allergy include bronchial asthma, allergic rhinitis, and conjunctivitis .

Recombinant Der f 1 protein is widely used in research and diagnostic applications. It serves as an antigen in enzyme-linked immunosorbent assays (ELISA) and Western blots to detect specific antibodies in patient samples . This helps in diagnosing mite allergies and understanding the immune response to this allergen.

The recombinant Der f 1 protein is typically stored at -20°C to maintain its stability. When stored properly, it can remain stable for up to five years in its frozen state and for one month in solution at room temperature .