Fra e 1.0101

Allergen Fra e 1.0101 is a protein belonging to the Ole e 1-like family, which is known for its role in pollen tube growth regulation. This allergen is derived from the ash tree (Fraxinus excelsior), a member of the Oleaceae family. The recombinant form of this allergen, referred to as Fra e 1.0101 Recombinant, has been extensively studied for its clinical significance in pollen allergy, particularly in Central Europe where ash pollen is a common allergen .

The cDNA encoding Fra e 1 was amplified using polymerase chain reaction (PCR) and subsequently cloned into Escherichia coli for sequencing. The recombinant allergen was then produced in the yeast Pichia pastoris. This method ensured the production of a properly folded and functional protein, which was crucial for subsequent immunological studies .

The recombinant Fra e 1.0101 was secreted into the extracellular medium of the yeast cultures. It was purified through a series of chromatographic steps to achieve a high degree of purity. The protein consists of 145 amino acids and exhibits significant identity with other allergens in the Oleaceae family, such as Syr v 1, Ole e 1, and Lig v 1 .

Studies have shown that IgE antibodies from patients sensitized to ash pollen bind to the recombinant Fra e 1 with a prevalence of 75%. This binding was confirmed through various immunological assays, including immunoblotting, ELISA, and histamine release tests. Additionally, skin prick tests demonstrated that 29 out of 30 ash-sensitized patients reacted positively to the recombinant allergen .

The recombinant Fra e 1.0101 has significant potential in the diagnosis and treatment of ash pollen allergies. Its ability to induce histamine release and its high prevalence of IgE binding make it a valuable tool for allergy testing. Furthermore, its production in Pichia pastoris ensures a consistent and reliable source of the allergen for clinical use .