Pen a 1.0101
Sf9 insect cells.
Greater than 95% as determined by SDS-PAGE.
Description
Recombinant Tropomyosin Pen a 1.0101 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 38kDa.
Pen a 1.0101 is expressed with a 10xHis tag and purified by proprietary chromatographic techniques.
Product Specs
Penaeus aztecus, or brown shrimp, contains Tropomyosin Pen a 1.0101, its only known major allergen. This panallergen is a highly conserved and soluble muscle protein characterized by an alpha-helical homodimeric, coiled-coil structure.
Recombinant Tropomyosin Pen a 1.0101, expressed in SF9 cells, is a glycosylated polypeptide chain with a calculated molecular mass of 38kDa. This protein is expressed with a 10xHis tag and purified using proprietary chromatographic techniques.
Pen a 1.0101 is supplied in a 20mM HEPES buffer with a pH of 7.9, 200mM NaCl, and 6M Urea.
For optimal storage, keep at 4°C if the entire vial will be used within 2-4 weeks. For longer storage periods, store frozen at -20°C. Avoid repeated freeze-thaw cycles.
Purity is determined to be greater than 95% as assessed by SDS-PAGE.
Sf9 insect cells.
Product Science Overview
Tropomyosin Pen a 1.0101 is a recombinant protein derived from the brown shrimp, Penaeus aztecus. It is a significant allergen and is known for its highly conserved and soluble muscle protein properties. This protein is of particular interest due to its role as a major allergen in shrimp, making it a critical subject of study for allergy research and diagnostics.
Tropomyosin Pen a 1.0101 is characterized by its alpha-helical homodimeric, coiled-coil structure. This structural configuration is essential for its function and stability. The recombinant form of this protein is produced in Sf9 insect cells and is glycosylated, resulting in a polypeptide chain with a calculated molecular mass of approximately 38 kDa .
The protein is expressed with a 10xHis tag, which facilitates its purification through proprietary chromatographic techniques. The final product is a sterile, filtered, colorless solution supplied in a buffer containing 20 mM HEPES, pH 7.9, 200 mM sodium chloride, and 6 M urea .
The recombinant Tropomyosin Pen a 1.0101 is produced using Sf9 insect cells, a common expression system for producing recombinant proteins. This system allows for proper folding and post-translational modifications, such as glycosylation, which are crucial for the protein’s functionality and stability.
The purification process involves multiple chromatographic steps to ensure a high degree of purity. The final product has a purity greater than 95%, as determined by SDS-PAGE .
Tropomyosin Pen a 1.0101 is primarily used in allergy research. As the major allergen in brown shrimp, it is a critical component in the development of diagnostic tests and therapeutic approaches for shrimp allergies. The recombinant form allows for consistent and reliable results in laboratory settings.
