MALD1
Major allergen Mal d 1, Ypr10 protein, MALD1, ypr10, Mal d 1.0108
Description
Recombinant MALD1 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 17,492 Dalton.
MALD1 purified by proprietary chromatographic techniques.
Product Specs
As a heat-sensitive allergen and ribonuclease, MALD1 belongs to the pathogenesis-related protein class. It exhibits homologous IgE epitopes with prominent birch pollen allergen Bet v 1 and hazelnut pollen allergen Cor a 1.
When produced in SF9 cells, recombinant MALD1 is a glycosylated polypeptide chain with a calculated molecular mass of 17,492 Daltons.
The purification of MALD1 is achieved through proprietary chromatographic methods.
The supplied formulation of MALD1 consists of 20mM HEPES buffer with a pH of 7.6, 250mM NaCl, and 20% glycerol.
2. Shows reactivity in immunodot assays utilizing positive and negative serum panels.
Major allergen Mal d 1, Ypr10 protein, MALD1, ypr10, Mal d 1.0108
Product Science Overview
Mal d 1 is a major allergen found in apples (Malus domestica) and is known to cause allergic reactions in individuals sensitive to birch pollen. This allergen is part of the pathogenesis-related protein class and is a ribonuclease. The recombinant form of this allergen, Mal d 1.0108, is produced for research and diagnostic purposes.
The recombinant Mal d 1 (Mal d 1.0108) is typically produced using the yeast Pichia pastoris. The protein is purified from the culture through multi-step chromatography, ensuring a purity of over 95% as confirmed by SDS-PAGE . The recombinant protein is formulated in a buffer containing 20mM Tris, 0.3M NaCl, pH 8.0, and is filtered through a 0.22μm filter to ensure sterility .
Mal d 1 is a heat-sensitive allergen, meaning its allergenic properties can be affected by thermal processing. The protein’s structure and IgE-binding epitopes can be analyzed using techniques such as NMR spectroscopy and SDS-PAGE . These analyses help in understanding the protein’s stability and its interaction with IgE antibodies.
Recombinant Mal d 1 is used in various research and diagnostic applications. It is employed in studies to understand the molecular basis of apple allergies and to develop diagnostic tests for detecting apple-specific IgE antibodies in allergic individuals. Additionally, it is used in the development of hypoallergenic apple varieties through genetic engineering.
