Growth Factors
CD Antigens
Enzymes
Viral Antigens
Recombinant Proteins
Natural Proteins
Monoclonal Antibodies
Cytokines
Polyclonal Antibodies
Neurotrophins
Chemokines
Hormones
Enzymes
Product List
PARP1 Human
Poly (ADP-Ribose) Polymerase 1 Human Recombinant
PARP1 Human Recombinant produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 354 amino acids (662-1014a.a.) and having a molecular mass of 39.6 kDa.
PARP1 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs 
Cat. No.
BT2769
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.
PARP2 Human
Poly (ADP-Ribose) Polymerase 2 Human Recombinant
PARP2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 376 amino acids (233-583a.a) and having a molecular mass of 42.5kDa. PARP2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2830
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.
POLL Human
Polymerase (DNA directed), Lambda Human Recombinant
POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.
POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
POLR2D Human
Polymerase II Polypeptide D Human Recombinant
POLR2D Human Recombinant produced in E.coli is a single,
non-glycosylated polypeptide chain containing 165 amino acids (1-142) and
having a molecular mass of 18.7kDa.POLR2D is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Shipped with Ice Packs
POLR2E Human
Polymerase II Polypeptide E Human Recombinant
POLR2E Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210) and having a molecular mass of 27.1kDa.
POLR2E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLR2E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
POLR2F Human
Polymerase II Polypeptide F Human Recombinant
POLR2F Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127) and having a molecular mass of 16.9kDa (Molecular size on SDS-PAGE will appear higher).
POLR2F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLR2F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
POLR2I Human
Polymerase II Polypeptide I Human Recombinant
POLR2I Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids (1-125 a.a.) and having a molecular mass of 17.0kDa.
POLR2I is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLR2I is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
POLR2J2 Human
Polymerase II Polypeptide J2 Human Recombinant
POLR2J2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-115) and having a molecular mass of 15.5kDa.
POLR2J2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLR2J2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
POLR3F Human
Polymerase III Polypeptide F Human Recombinant
POLR3F Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316) and having a molecular mass of 38.1kDa.
POLR3F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLR3F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
POLR3K Human
Polymerase III Polypeptide K Human Recombinant
POLR3K Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 131 amino acids (1-108 a.a) and having a molecular mass of 14.7kDa.
POLR3K is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLR3K is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3290
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.
Introduction
Definition and Classification
Polymerases are enzymes that synthesize long chains of polymers or nucleic acids. They play a crucial role in the replication and transcription of genetic material. There are two main types of polymerases:
Biological Properties
Key Biological Properties:
- DNA Polymerase: Essential for DNA replication, repair, and recombination. It adds nucleotides to the 3’ end of a DNA strand, ensuring accurate copying of genetic information .
- RNA Polymerase: Catalyzes the synthesis of RNA from a DNA template during transcription .
Expression Patterns and Tissue Distribution:
- DNA polymerases are ubiquitously expressed in all dividing cells, with higher expression in tissues with rapid cell turnover, such as the bone marrow and gastrointestinal tract .
- RNA polymerases are also widely expressed, with specific types (Pol I, Pol II, Pol III) having distinct roles in different tissues .
Biological Functions
Modes of Action
Mechanisms with Other Molecules and Cells:
- DNA polymerases interact with various proteins involved in the replication fork, such as helicases and primases .
- RNA polymerases interact with transcription factors and other regulatory proteins to initiate and regulate transcription .
Binding Partners and Downstream Signaling Cascades:
Regulatory Mechanisms
Applications
Biomedical Research:
- Polymerases are essential tools in molecular biology techniques such as PCR (Polymerase Chain Reaction) and DNA sequencing .
Diagnostic Tools:
- PCR-based diagnostic tests for infectious diseases and genetic disorders rely on the activity of DNA polymerases .
Therapeutic Strategies:
Role in the Life Cycle
Throughout Development to Aging and Disease:
- DNA polymerases are crucial for cell division and development, ensuring accurate DNA replication .
- RNA polymerases are involved in the transcription of genes necessary for growth, development, and response to environmental changes .
- Dysregulation of polymerase activity can lead to diseases such as cancer and genetic disorders .
