Enzymes
Product List
GBA Human
Beta-Glucocerebrosidase Human Recombinant
GBA3 Human
Glucosidase Beta Acid 3 Human Recombinant
GBA3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Glucosidases are a group of glycoside hydrolase enzymes that catalyze the hydrolysis of glycosidic bonds in complex sugars. They are categorized under the EC number 3.2.1 and are involved in breaking down complex carbohydrates such as starch and glycogen into their monomers . Glucosidases can be classified into alpha-glucosidases and beta-glucosidases based on their substrate specificity and the type of glycosidic bond they hydrolyze .
Key Biological Properties: Glucosidases are essential for the metabolism of carbohydrates. They hydrolyze terminal non-reducing glucosyl residues from glycosides and oligosaccharides .
Expression Patterns and Tissue Distribution: Glucosidases are expressed in various tissues across different organisms. For instance, alpha-glucosidase is located in the brush border of the small intestine, while beta-glucosidase is found in lysosomes and other cellular compartments .
Primary Biological Functions: Glucosidases play a crucial role in carbohydrate metabolism by breaking down complex sugars into simpler forms that can be absorbed and utilized by the body .
Role in Immune Responses and Pathogen Recognition: Glucosidases are involved in the degradation of glycoproteins and glycolipids, which can influence immune responses and pathogen recognition .
Mechanisms with Other Molecules and Cells: Glucosidases hydrolyze glycosidic bonds through a mechanism involving two carboxylic acid-containing amino acids in the catalytic site, acting either as an acid or a base .
Binding Partners and Downstream Signaling Cascades: The enzyme binds to specific substrates and catalyzes the cleavage of glycosidic bonds, leading to the release of glucose or other monosaccharides .
Regulatory Mechanisms Controlling Expression and Activity: The expression of glucosidase genes is regulated by various factors, including carbon catabolite repression, which controls enzyme production based on the availability of glucose .
Transcriptional Regulation and Post-Translational Modifications: Transcriptional regulation involves the activation or repression of glucosidase genes in response to metabolic needs. Post-translational modifications, such as phosphorylation, can also affect enzyme activity .
Biomedical Research: Glucosidases are used in research to study carbohydrate metabolism and related disorders .
Diagnostic Tools: Enzyme assays involving glucosidases are used to diagnose metabolic diseases such as Gaucher’s disease and Pompe disease .
Therapeutic Strategies: Glucosidase inhibitors are used as therapeutic agents to manage conditions like type 2 diabetes by delaying carbohydrate absorption .
Role Throughout the Life Cycle: Glucosidases are involved in various stages of the life cycle, from development to aging. They play a role in the digestion of dietary carbohydrates, the metabolism of glycogen, and the degradation of cellular components .
From Development to Aging and Disease: During development, glucosidases are crucial for energy production and growth. In aging, their activity can influence the progression of metabolic diseases .
