Enzymes
Product List
TGM2 Human
Tissue Transglutaminase Human Recombinant
TGM2 Human, Sf9
Tissue Transglutaminase Human Recombinant, Sf9
Tissue Transglutaminase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83 kDa. tTG is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating.
TGM2 Mouse
Tissue Transglutaminase Mouse Recombinant
TGM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Transglutaminase 2 (TGM2), also known as tissue transglutaminase (tTG), is a multifunctional enzyme belonging to the transglutaminase family. It is a calcium-dependent enzyme that catalyzes the formation of covalent bonds between proteins by cross-linking glutamine and lysine residues . TGM2 is classified under the enzyme commission number EC 2.3.2.13.
Key Biological Properties: TGM2 is a ubiquitously expressed enzyme with multiple enzymatic activities, including transamidation, deamidation, GTPase, and protein disulfide isomerase activities .
Expression Patterns and Tissue Distribution: TGM2 is expressed in various tissues, including the central nervous system, liver, heart, and skin . It is found in the cytoplasm, nucleus, and extracellular matrix, indicating its diverse functional roles .
Primary Biological Functions: TGM2 plays a crucial role in cell growth, differentiation, apoptosis, and tissue repair . It is involved in the formation of the extracellular matrix and stabilization of the cytoskeleton .
Role in Immune Responses and Pathogen Recognition: TGM2 is implicated in immune responses by modulating inflammation and facilitating the clearance of apoptotic cells . It also plays a role in pathogen recognition and defense mechanisms .
Mechanisms with Other Molecules and Cells: TGM2 interacts with various molecules, including proteins, nucleotides, and ions. It catalyzes the cross-linking of proteins through transamidation reactions, leading to the formation of stable protein complexes .
Binding Partners and Downstream Signaling Cascades: TGM2 binds to several proteins, including fibronectin, integrins, and histones . It is involved in multiple signaling pathways, such as the Wnt/β-catenin pathway, which regulates cell proliferation and differentiation .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of TGM2 are regulated at multiple levels, including transcriptional and post-translational modifications . Calcium ions and GTP/GDP binding play critical roles in modulating TGM2 activity .
Transcriptional Regulation and Post-Translational Modifications: TGM2 expression is regulated by various transcription factors and signaling molecules. Post-translational modifications, such as phosphorylation and acetylation, further modulate its activity and stability .
Biomedical Research: TGM2 is widely studied in biomedical research for its role in various physiological and pathological processes .
Diagnostic Tools: TGM2 serves as a biomarker for certain diseases, including celiac disease and cancer .
Therapeutic Strategies: Targeting TGM2 has therapeutic potential in treating conditions such as fibrosis, cancer, and neurodegenerative diseases .
Role Throughout the Life Cycle: TGM2 is involved in various stages of the life cycle, from development to aging . It plays a role in embryonic development, tissue homeostasis, and aging-related processes .
From Development to Aging and Disease: During development, TGM2 contributes to tissue formation and differentiation. In aging, it is associated with tissue repair and fibrosis. Dysregulation of TGM2 activity is linked to various diseases, including cancer and neurodegenerative disorders .
